• Users Online: 1496
  • Home
  • Print this page
  • Email this page
RESEARCH AND REPORTS
Year : 2014  |  Volume : 9  |  Issue : 1  |  Page : 33-40

Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation


1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu; Department of Anatomy, Luzhou Medical College, Luzhou\, Sichuan Province, China
2 Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou\, Sichuan Province, China
3 Department of Anatomy, Luzhou Medical College, Luzhou\, Sichuan Province, China

Correspondence Address:
Yu Zheng
Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1673-5374.125327

Rights and Permissions

Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, microtubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the supernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1425    
    Printed47    
    Emailed0    
    PDF Downloaded312    
    Comments [Add]    
    Cited by others 4    

Recommend this journal