Polysaccharides from Angelica sinensis alleviate neuronal cell injury caused by oxidative stress
Tao Lei1, Haifeng Li2, Zhen Fang1, Junbin Lin1, Shanshan Wang1, Lingyun Xiao2, Fan Yang2, Xin Liu2, Junjian Zhang1, Zebo Huang2, Weijing Liao1
1 Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China
2 School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China
Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, 169# Donghu Road, Wuhan 430071, Hubei Province
Source of Support: This study was supported by the National Natural Science Foundation of China, No. 81072917 and 81274048., Conflict of Interest: None
Angelica sinensis has antioxidative and neuroprotective effects. In the present study, we aimed to determine the neuroprotective effect of polysaccharides isolated from Angelica sinensis. In a preliminary experiment, Angelica sinensis polysaccharides not only protected PC12 neuronal cells from H 2 O 2 -induced cytotoxicity, but also reduced apoptosis and intracellular reactive oxygen species levels, and increased the mitochondrial membrane potential induced by H 2 O 2 treatment. In a rat model of local cerebral ischemia, we further demonstrated that Angelica sinensis polysaccharides enhanced the antioxidant activity in cerebral cortical neurons, increased the number of microvessels, and improved blood flow after ischemia. Our findings highlight the protective role of polysaccharides isolated from Angelica sinensis against nerve cell injury and impairment caused by oxidative stress.