• Users Online: 948
  • Home
  • Print this page
  • Email this page
TECHNICAL UPDATES
Year : 2014  |  Volume : 9  |  Issue : 5  |  Page : 513-518

Negative regulation of miRNA-9 on oligodendrocyte lineage gene 1 during hypoxic-ischemic brain damage


Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing, China

Correspondence Address:
Hong Cui
Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050
China
Login to access the Email id

Source of Support: Funding: This study was supported by the National Natural Science Foundation of China, No. 81241022; the Beijing Municipal Natural Science Foundation in China, No. 7122045, 7072023., Conflict of Interest: None


DOI: 10.4103/1673-5374.130077

Rights and Permissions

Oligodendrocyte lineage gene 1 plays a key role in hypoxic-ischemic brain damage and myelin repair. miRNA-9 is involved in the occurrence of many related neurological disorders. Bioinformatics analysis demonstrated that miRNA-9 complementarily, but incompletely, bound oligodendrocyte lineage gene 1, but whether miRNA-9 regulates oligodendrocyte lineage gene 1 remains poorly understood. Whole brain slices of 3-day-old Sprague-Dawley rats were cultured and divided into four groups: control group; oxygen-glucose deprivation group (treatment with 8% O2 + 92% N2 and sugar-free medium for 60 minutes); transfection control group (after oxygen and glucose deprivation for 60 minutes, transfected with control plasmid) and miRNA-9 transfection group (after oxygen and glucose deprivation for 60 minutes, transfected with miRNA-9 plasmid). From the third day of transfection, and with increasing culture days, oligodendrocyte lineage gene 1 expression increased in each group, peaked at 14 days, and then decreased at 21 days. Real-time quantitative PCR results, however, demonstrated that oligodendrocyte lineage gene 1 expression was lower in the miRNA-9 transfection group than that in the transfection control group at 1, 3, 7, 14, 21 and 28 days after transfection. Results suggested that miRNA-9 possibly negatively regulated oligodendrocyte lineage gene 1 in brain tissues during hypoxic-ischemic brain damage.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1301    
    Printed18    
    Emailed0    
    PDF Downloaded226    
    Comments [Add]    
    Cited by others 2    

Recommend this journal