Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
Xiao-ge Yan1, Bao-hua Cheng2, Xin Wang2, Liang-cai Ding2, Hai-qing Liu3, Jing Chen2, Bo Bai M.D. 2
1 Xinxiang Medical University, Xinxiang, Henan Province, China
2 Neurobiology Institute, Jining Medical University, Jining, Shandong Province, China
3 Taishan Medical University, Taian, Shandong Province, China
Neurobiology Institute, Jining Medical University, Jining, Shandong Province
Source of Support: This study was financially supported by the National Natural Science Foundation of China, No. 30971081, 31271243, 81070961 and 81241052; and the Natural Science Foundation of Shandong Province of China, No. ZR2011CM027 and 2012GGA08100.
Yan XG, Cheng BH, Wang X, Ding LC, Liu HQ, Chen J, Bai B (2015) Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury. Neural Regen Res 10(5):766-771.
, Conflict of Interest: None
Apelin-13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 (0.1 µg/g) was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immunohistochemical staining showed that compared with the cerebral ischemia/reperfusion group, infarct volume and apoptotic cell number at the ischemic penumbra region were decreased in the Apelin-13 treatment group. Additionally, Apelin-13 treatment increased Bcl-2 immunoreactivity and decreased caspase-3 immunoreactivity. Our findings suggest that Apelin-13 is neuroprotective against cerebral ischemia/reperfusion injury through inhibition of neuronal apoptosis.