|Year : 2016 | Volume
| Issue : 3 | Page : 460-464
Neuroprotection against vascular dementia after acupuncture combined with donepezil hydrochloride: P300 event related potential
Qiang Liu1, Xiu-juan Wang2
, Zhe-cheng Zhang3, Rong Xue4, Ping Li5, Bo Li1
1 First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China
2 Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine, Tianjin, China
3 Tianjin Third Central Hospital, Tianjin, China
4 General Hospital of Tianjin Medical University, Tianjin, China
5 Tianjin Quanjian International Cancer Hospital, Tianjin, China
|Date of Acceptance||11-Jan-2016|
|Date of Web Publication||1-Apr-2016|
Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine, Tianjin
Source of Support: None, Conflict of Interest: None
Acupuncture can be used to treat various nervous system diseases. Here, 168 vascular dementia patients were orally administered donepezil hydrochloride alone (5 mg/day, once a day for 56 days), or combined with acupuncture at Shenting (DU24), Tianzhu (BL10), Sishencong (Extra), Yintang (Extra), Renzhong (DU26), Neiguan (PC6), Shenmen (HT7), Fengchi (GB20), Wangu (GB12) and Baihui (DU20) (once a day for 56 days). Compared with donepezil hydrochloride alone, P300 event related potential latency was shorter with an increased amplitude in patients treated with donepezil hydrochloride and acupuncture. Mini-Mental State Examination score was also higher. Moreover, these differences in P300 latency were identified within different infarcted regions in patients treated with donepezil hydrochloride and acupuncture. These findings indicate that acupuncture combined with donepezil hydrochloride noticeably improves cognitive function in patients with vascular dementia, and exerts neuroprotective effects against vascular dementia.
Keywords: nerve regeneration; vascular dementia; acupuncture; donepezil hydrochloride; event related potential; cognitive function; infarct focus; neural regeneration
|How to cite this article:|
Liu Q, Wang Xj, Zhang Zc, Xue R, Li P, Li B. Neuroprotection against vascular dementia after acupuncture combined with donepezil hydrochloride: P300 event related potential. Neural Regen Res 2016;11:460-4
|How to cite this URL:|
Liu Q, Wang Xj, Zhang Zc, Xue R, Li P, Li B. Neuroprotection against vascular dementia after acupuncture combined with donepezil hydrochloride: P300 event related potential. Neural Regen Res [serial online] 2016 [cited 2020 Apr 9];11:460-4. Available from: http://www.nrronline.org/text.asp?2016/11/3/460/179059
Author contributions: QL provided data, ensured the integrity of the data, and participated in study conception and design. XJW participated in data analysis, and paper writing. PL provided technical support. ZCZ and RX were in charge of paperauthorization. All authors approved the final version of the paper.
Conflicts of interest: None declared.
This paper was screened twice using CrossCheck to verify originality before publication.
Peer review: This paper was double-blinded and stringently reviewed by international expert reviewers.
| Introduction|| |
Vascular dementia is strongly associated with cortical lesions, and particularly ischemia in the left cortex, thalamus and, hippocampus (Liu et al., 2014). Vascular dementia is also related to bilateral multiple cerebral infarction, ischemic infarcts in major brain regions, and large-area brain damage (Gorelick, 1997). It is mainly induced by cerebrovascular disease, and therefore shares many common risk factors with stroke e.g., hypertension (Qiu et al., 2005; Paglieri et al., 2008), diabetes (Araki, 2010; Fei et al., 2013), hyperlipemia (Purandare, 2009; Solomon et al., 2009), smoking (Mazzone et al., 2010; Gons et al., 2011), and drinking (Lobo et al., 2010; Weyerer et al., 2011). Medication for Alzheimer's disease and mild cognitive impairment is administered for vascular dementia, but the clinical significance of these medicines is uncertain (Dichgans et al., 2008). Complementary and alternative therapies such as acupuncture, exercise, and cognitive therapy have obtained certain therapeutic effects (Avila et al., 2004; Ding et al., 2006). Donepezil hydrochloride is a reversible, specific, long-acting acetylcholine esterase inhibitor that can effectively and selectively suppress acetylcholine degradation in the central nervous system, increase acetylcholine concentration in the synaptic cleft, and improve memory and mental status in vascular dementia patients (Meyer et al., 2002; Naik et al., 2009; Liu et al., 2012).
Acupuncture is one of the classical methods of traditional Chinese medicine, and can regulate learning and memory, the stress response, and emotion (Toriizuka et al., 1999; Yang et al., 2002; dos Santos Jr et al., 2008). Animal experiments show that acupuncture can treat Parkinson's disease by inhibiting dopamine decline (Kim et al., 2005), restore memory ability in rats with cerebral ischemia associated with diabetes (Jing et al., 2007, 2008), and improve cognitive dysfunction in age-accelerated mice (Cheng et al., 2008). Evidence-based medicine indicates that acupuncture combined with cognitive training effectively improves cognitive function, with the effects of acupuncture better than drug treatment alone (Zhou et al., 2013).
Sutton et al. (1965) first reported P300 event related potentials. The P300 event related potential defines electroencephalogram changes during cognitive processing (e.g., attention, memory, and thinking) of objective events. Latency reflects the speed of event coding, classification, and identification, and objectively reflects the brain's cognitive and decision-making functions. Prolonged P300 latency indicates cognitive dysfunction induced by various encephalopathies. Amplitude and information processing are associated with the extent of effective resource mobilization. Decreased amplitude indicates reduced degree of effective resource mobilization during information processing (McCarthy and Donchin, 1981). Gaillard (1988) suggested that the P300 is the reaction advanced processing sensory endogenous component: it is a quantifiable electrophysiological indicator that objectively reflects the sensitive cognitive function, displays the degree of cognitive impairment, and monitors cognitive dysfunction (Gummow et al., 1986; Damulin et al., 2008). The Mini-Mental State Examination (MMSE) exhibits high specificity for measuring vascular dementia (Sun and Qin, 2011). Here, we used the P300 event related potential and MMSE to measure the protective effect of acupuncture combined with donepezil hydrochloride for treating vascular dementia after stroke.
| Subjects and Methods|| |
A total of 272 stroke patients treated in the Department of Acupuncture and Moxibustion, Tianjin Nankai Hospital (China) and the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine (China) from January to December 2012 were recruited for this study. The diagnostic criteria were in accordance with the vascular dementia criteria formulated by the National Institute of Neurological Disease and Stroke and Association Internationale pour la Recherche er l'Enseignement en Neurosciences (NINDS-AIREN) (Rockwood et al., 2000). (1) Dementia and decline in cognitive capacity: memory impairment and one or more cognitive impairments (orientation, attention, language, visual-spatial function, executive function, motion control, and enforcement function); (2) cerebrovascular disease: focal neurological signs and relevant cerebrovascular disorders detected by CT/MRI; (3) dementia occurred within 3 months after stroke; (4) at least one or two of the following items: sudden cognitive decline or wave-like, ladder-like progression of cognitive impairment.
The inclusion criteria were: (1) vascular dementia criteria formulated by NINDS-AIREN; (2) MMSE score (Zhou et al., 2013) ≤ 23; (3) life and social dysfunction (not only induced by physical dysfunction), activities of daily living (Tian et al., 2000) > 20; (4) Hachinski Ischemic Scale (Tian et al., 2000) ≥ 7; (5) cognitive dysfunction appeared within 3 months after cerebrovascular disease; (6) mild or moderate National Institutes of Health Stroke Scale (NIHSS).
The exclusion criteria were: (1) patients exhibited unconsciousness, delirium, neurosis, severe aphasia, or obvious sensorimotor disorders, but did not show evidence of this on neuropsychological tests; (2) patients had systemic diseases that can cause memory or cognitive dysfunction, or other brain diseases such as Alzheimer's disease; (3) patients had taken antiepileptic drugs or antidepressants, before the stroke or dementia; (4) the patient or their family members did not agree to and did not complete the initial neuropsychological assessment; (5) patients were uncooperative and had poor compliance despite repeated explanations by the clinician; and (6) the patient's condition worsened during the study and emergency measures had to be taken. In addition, those (7) that dropped out; (8) did not receive regular treatment or did not have complete data; or (9) suffered from severe adverse events or complications and could not finish the study.
Of 272 vascular dementia patients, 168 patients finished the trial, including 106 males and 62 females. They were an average of 56 years old, ranging 45-55 years old (32 cases), 56-65 years old (78 cases), and 66-75 years old (58 cases). The disease course was between 3 months and 3 years (1.6 years on average). In addition, 95 cases had a history of hypertension, and 49 had a history of diabetes. According to CT or magnetic resonance angiography (MRA), the infarcted regions were the parietal lobe, frontal lobe, temporal lobe, occipital lobe, basal ganglia, internal capsule, and thalamus. Infarction was detected in the basal ganglia, internal capsule, and thalamus in 89 cases (unilateral in 49 cases, 55%; bilateral in 40 cases, 45%), in the parietal lobe and occipital lobe in 39 cases (unilateral in 20 cases, 51%; bilateral in 19 cases, 49%), and in the frontal lobe and temporal lobe in 40 cases (unilateral in 17 cases, 42%; bilateral in 23 cases, 58%).
All patients were equally and randomly divided into control and treatment groups. No significant differences in MMSE score, NIHSS score, Barthel index, and infarct region were identified between the two groups (P > 0.05), and the groups were comparable ([Table 1]).
Treatment with donepezil hydrochloride
Patients in the control and treatment groups orally took donepezil hydrochloride (trade name: Aricept; Eisai China Inc., Suzhou, Jiangsu Province, China; Approval No. H20050978): 5 mg/day, once a day, with 7 days as a course, for 8 courses. In accordance with the physical and laboratory examination results, drug treatments for improving circulation, regulating blood glucose, blood pressure, and blood lipids were executed.
Simultaneously, the patients in the treatment group were subjected to acupuncture at Shenting (DU24), Tianzhu (BL10) (bilateral), Sishencong (Extra), Yintang (Extra), Renzhong (DU26), Neiguan (PC6) (bilateral), Shenmen (HT7) (bilateral), Fengchi (GB20) (bilateral), Wangu (GB12) (bilateral), and Baihui (DU20). In accordance with Acupuncture and Moxibustion (Lu, 2002), patients in the supine position received acupuncture at Fengchi (GB20), Wangu (GB12), and Tianzhu (BL10) using a stainless steel needle (0.30 mm × 40 mm in diameter; Suzhou Medical Sino-foreign Joint Venture Suzhou Hua Tuo Medical Instruments Co., Ltd., Suzhou, China), with twirling at > 180 r/min for 1 minute until the patients felt soreness, numbness, and distention. Subsequently, the needle was inserted at Baihui (DU20) and Shenting (DU24) for 0.5-0.8 cun, and twirled at > 180 r/min for 1 minute until the local sensation of soreness, numbness, or distention was detected in the back of the head. The needle was then inserted at Shenting (DU24) and Sishencong (Extra) for 0.5 cun and twirled at > 180 r/min for 1 minute, and at Yintang (Extra) towards the nasal root for 0.3-0.5 cun with light twirling but no lifting or thrusting. The needle was obliquely inserted at Renzhong (DU26) towards the nasal septum for 0.3-0.5 cun, and the needling manipulation of heavy bird-peck used until the eyeballs were wet or tears appeared. Neiguan (PC6) and Shenmen (HT7) were perpendicularly manipulated for 0.5 cun using a mild reinforcing and attenuating method combined with a twirling, lifting, and thrusting method for 1 minute. Acupuncture in the treatment group was performed once a day, with 14 days as a course, for a total of 4 courses. The control group did not undergo any acupuncture.
P300 event related potential measured using an electromyogram evoked potentiometer
The P300 event related potential was measured using a NDI-200 electromyogram evoked potentiometer (Dantec Dynamics A/S, Copenhagen, Denmark). The electrodes were placed on patients in the supine position in accordance with International 10-20 system (Zhou, 2001). Recording electrodes (G-) were placed on Fz, Cz, Pz, etc. Reference electrodes (G+) were placed on the ears (A1 or A2). A groundwire was placed on the forehead. Auditory stimulation was performed using an oddball sequence, which is a sequence of pure tone composed of target stimuli (target, T) with a frequency of 2,000 Hz, and non-target stimuli (non-target, NT) with a frequency of 1,000 Hz. The change in tone length ranged from 5 to 10 ms (50-100 ms in duration). The sound pressure level increased by 60 dB (on the basis of subjective hearing thresholds) with 2-second intervals.
The MMSE determined the cognitive function of patients. MMSE is a 30-point questionnaire that contains seven categories: orientation to time, orientation to place, registration, attention calculation, recall, language, and repetition. The following cut-off levels are used to classify the severity of cognitive impairment: no cognitive impairment = 27-30; mild cognitive impairment = 21-26; moderate cognitive impairment = 10-20; and severe cognitive impairment = 0-10.
Data were processed using SPSS 17.0 software (SPSS, Chicago, IL, USA) and expressed as the mean ± SD. Numeration data were compared between the two groups using the chi-square test. Measurement data were analyzed using analysis of variance with a randomized block design. Two-sample t-test was used if a normal distribution and homogenous variance were observed. The rank sum test was used if heterogeneity of variance was observed. A value of P < 0.05 was considered statistically significant.
| Results|| |
Improvement in P300 event related potential in vascular dementia patients after acupuncture combined with donepezil hydrochloride
In both groups after treatment, the P300 latency shortened while the amplitude increased at different sites (Fz, Cz, and Pz). However, the latency was shorter and amplitude greater in the treatment group compared with the control group (P < 0.05). In the treatment group, no significant difference in latency and amplitude was detected among the three sites (Fz, Cz, and Pz) (P > 0.05; [Table 2]).
|Table 2: Change in latency (ms) and amplitude (μV) of P300 event related potential after treatment with acupuncture and donepezil hydrochloride|
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Highly increased MMSE score in vascular dementia patients after acupuncture combined with donepezil hydrochloride
MMSE score was examined in the treatment and control groups after treatment (P < 0.01 or P < 0.05). MMSE score was significantly higher in the treatment group than the control group (P < 0.05; [Table 3]).
|Table 3: Change in MMSE score in vascular dementia patients after acupuncture combined with donepezil hydrochloride|
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Change in P300 event related potential in different infarcted regions of vascular dementia patients before and after acupuncture combined with donepezil hydrochloride
In the treatment group, the P300 latency was significantly shortened and the amplitude significantly prolonged (P < 0.01). No significant difference in latency and amplitude was detected in 19 patients with frontal lobe and temporal lobe infarction in the control group after treatment (P > 0.05). In addition, the latency was shortened (P < 0.01), but amplitude not significantly altered in 46 patients with basal ganglia infarction in the control group after treatment (P > 0.05). However, the amplitude was significantly increased and latency significantly shortened in 19 patients with parietal lobe and occipital lobe infarction in the control group (P < 0.01; [Table 4]).
|Table 4: Change in latency (ms) and amplitude (μV) in different infarcted regions of vascular dementia patients before and after acupuncture combined with donepezil hydrochloride|
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| Discussion|| |
Cognitive dysfunction mainly presents with a prolonged P300 latency and decreased amplitude in stroke patients (Suzuki and Hoshiyama, 2011). However, our results show no significant difference in latency and amplitude at Fz, Cz, and Pz before and after treatment in the treatment group. The P300 event related potential reflects brain function. Xu et al. (2010) confirmed that acetylcholine, serotonin, and norepinephrine can regulate P300 latency and amplitude. Furthermore, acupuncture regulates cholinesterase inhibitors, serotonin, and noradrenaline after dementia.
In conclusion, acupuncture combined with donepezil hydrochloride effectively improves cognitive function in vascular dementia patients. Thus, our combined therapy has noticeable advantages in improving cognitive function and protecting against vascular dementia compared with donepezil hydrochloride alone.
| References|| |
Araki A (2010) Dementia and insulin resistance in patients with diabetes mellitus. Nihon Rinsho 68:569-574.
Avila R, Bottino CM, Carvalho IA, Santos CB, Seral C, Miotto EC (2004) Neuropsychological rehabilitation of memory deficits and activities of daily living in patients with Alzheimer's disease: a pilot study. Braz J Med Biol Res 37:1721-1729.
Cheng H, Yu J, Jiang Z, Zhang X, Liu C, Peng Y, Chen F, Qu Y, Jia Y, Tian Q, Xiao C, Chu Q, Nie K, Kan B, Hu X, Han J (2008) Acupuncture improves cognitive deficits and regulates the brain cell proliferation of SAMP8 mice. Neurosci Lett 432:111-116.
Damulin IV, Koberskaya NN, Mkhitaryan éA (2008) Effects of cerebrolysin on moderate cognitive impairments in cerebral vascular insufficiency (a clinical-electrophysiological study). Neurosci Behav Physiol 38:639-645.
Dichgans M, Markus HS, Salloway S, Verkkoniemi A, Moline M, Wang Q, Posner H, Chabriat HS (2008) Donepezil in patients with subcortical vascular cognitive impairment: a randomised double-blind trial in CADASIL. The Lancet Neurology 7:310-318.
Ding X, Yu J, Yu T, Fu Y, Han J (2006) Acupuncture regulates the aging-related changes in gene profile expression of the hippocampus in senescence-accelerated mouse (SAMP10). Neurosci Lett 399:11-16.
dos Santos Jr JG, Kawano F, Nishida MM, Yamamura Y, Mello LE, Tabosa Â (2008) Antidepressive-like effects of electroacupuncture in rats. Physiol Behav 93:155-159.
Fei M, Yan Ping Z, Ru Juan M, Ning Ning L, Lin G (2013) Risk factors for dementia with type 2 diabetes mellitus among elderly people in China. Age Ageing 42:398-400.
Gaillard AW (1988) Problems and paradigms in ERP research. Biol Psychol 26:91-109.
Gons RA, van Norden AG, de Laat KF, van Oudheusden LJ, van Uden IW, Zwiers MP, Norris DG, de Leeuw FE (2011) Cigarette smoking is associated with reduced microstructural integrity of cerebral white matter. Brain 134:2116-2124.
Gorelick PB (1997) Status of risk factors for dementia associated with stroke. Stroke 28:459-463.
Gummow LJ, Dustman RE, Keaney RP (1986) Cerebrovascular accident alters P300 event-related potential characteristics. Electroencephalogr Clin Neurophysiol 63:128-137.
Jing XH, Chen SL, Shi H, Cai H, Jin ZG (2008) Electroacupuncture restores learning and memory impairment induced by both diabetes mellitus and cerebral ischemia in rats. Neurosci Lett 443:193-198.
Jing XH, Cai H, Shi H, Chen SL, Lu B, Jin ZG (2007) Effect of acupuncture on learning-memory ability in diabetic rats with concomitant cerebral ischemia-reperfusion injury. Zhen Ci Yan Jiu 32:105-110.
Kim YK, Lim HH, Song YK, Lee HH, Lim S, Han SM, Kim CJ (2005) Effect of acupuncture on 6-hydroxydopamine-induced nigrostratal dopaminergic neuronal cell death in rats. Neurosci Lett 384:133-138.
Liu FX, Niu K, Xue GP (2014) The therapeutic progress of vascular dementia with modern medicine and chinese traditional treatment. Shenjing Yaoli Xuebao 6:29-33.
Liu YH, Li SW, Zheng QL (2012) Effects of bushen jiannao recipe on the content of acetylcholine and the hippocampal ERK1 and ERK2 protein expressions of vascular dementia rats. Zhongguo Zhong Xi Yi Jie He Za Zhi 32:504-509.
Lobo E, Dufouil C, Marcos G, Quetglas B, Saz P, Guallar E, Lobo A, ZARADEMP Workgroup (2010) Is there an association between low-to-moderate alcohol consumption and risk of cognitive decline? Am J Epidemiol 172:708-716.
Lu SK (2002) Acupuncture and Moxibustion. Beijing: China Press of Traditional Chinese Medicine.
Mazzone P, Tierney W, Hossain M, Puvenna V, Janigro D, Cucullo L (2010) Pathophysiological impact of cigarette smoke exposure on the cerebrovascular system with a focus on the blood-brain barrier: expanding the awareness of smoking toxicity in an underappreciated area. Int J Environ Res Public Health 7:4111-4126.
McCarthy G, Donchin E (1981) A metric for thought: a comparison of P300 latency and reaction time. Science 211:77-80.
Meyer JS, Chowdhury MH, Xu G, Li YS, Quach M (2002) Donepezil treatment of vascular dementia. Ann N Y Acad Sci 977:482-486.
Naik RS, Hartmann J, Kiewert C, Duysen EG, Lockridge O, Klein J (2009) Effects of rivastigmine and donepezil on brain acetylcholine levels in acetylcholinesterase-deficient mice. J Pharm Pharm Sci 12:79-85.
Paglieri C, Bisbocci D, Caserta M, Rabbia F, Bertello C, Canadè A, Veglio F (2008) Hypertension and cognitive function. Clin Exp Hypertens 30:701-710.
Purandare N (2009) Preventing dementia: role of vascular risk factors and cerebral emboli. Br Med Bull 91:49-59.
Qiu C, Winblad B, Fratiglioni L (2005) The age-dependent relation of blood pressure to cognitive function and dementia. Lancet Neurol 4:487-499.
Rockwood K, Wentzel C, Hachinski V, Hogan DB, MacKnight C, McDowell I (2000) Prevalence and outcomes of vascular cognitive impairment. Vascular Cognitive Impairment Investigators of the Canadian Study of Health and Aging. Neurology 54:447-451.
Solomon A, Kivipelto M, Wolozin B, Zhou J, Whitmer RA (2009) Midlife serum cholesterol and increased risk of Alzheimer's and vascular dementia three decades later. Dement Geriatr Cogn Disord 28:75-80.
Sun YC, Qin B (2011) The application of Montreal cognitive assessment in amnestic mild cognitive impairment. Zhongguo Shenjing Mianyi Xue yu Shenjing Bing Xue Zazhi 18:91-97.
Sutton S, Braren M, Zubin J, John ER (1965) Evoked-potential correlates of stimulus uncertainty. Science 150:1187-1188.
Suzuki M, Hoshiyama M (2011) Difference in P300 response between hemi-field visual stimulation. Neurol Sci 32:603-608.
Tian JZ, Han MX, Tu JW, Yang CZ, Yang HM, Zou YH, Liu H (2000) TCM diagnosis,differentiation,and criteria of clinical effectiveness in treating vascular dementia. Beijing Zhongyiyao Daxue Xuebao 23:16-25.
Toriizuka K, Okumura M, Iijima K, Haruyama K, Cyong JC (1999) Acupuncture inhibits the decrease in brain catecholamine contents and the impairment of passive avoidance task in ovariectomized mice. Acupunct Electrother Res 24:45-57.
Weyerer S, Schäufele M, Wiese B, Maier W, Tebarth F, van den Bussche H, Pentzek M, Bickel H, Luppa M, Riedel-Heller SG, German AgeCoDe Study group (German Study on Ageing, Cognition and Dementia in Primary Care Patients) (2011) Current alcohol consumption and its relationship to incident dementia: results from a 3-year follow-up study among primary care attenders aged 75 years and older. Age Ageing 40:456-463.
Xu Q, Zhou Y, Li YS, Cao WW, Lin Y, Pan YM, Chen SD (2010) Diffusion tensor imaging changes correlate with cognition better than conventional MRI findings in patients with subcortical ischemic vascular disease. Dement Geriatr Cogn Disord 30:317-326.
Yang CH, Lee BB, Jung HS, Shim I, Roh PU, Golden GT (2002) Effect of electroacupuncture on response to immobilization stress. Pharmacol Biochem Behav 72:847-855.
Zhou JH (2001) EEG interpretation step by step. Beijing: Science Press.
Zhou L, Zhang YL, Cao YJ, Hu H (2013) Treating vascular mild cognitive impairment by acupuncture: a systematic review of randomized controlled trials. Zhongguo Zhong Xi Yi Jie He Za Zhi 33:1626-1630.
[Table 1], [Table 2], [Table 3], [Table 4]
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