|Year : 2017 | Volume
| Issue : 12 | Page : 2035-2044
Neural computational modeling reveals a major role of corticospinal gating of central oscillations in the generation of essential tremor
Hong-en Qu1, Chuanxin M Niu2, Si Li1, Man-zhao Hao1, Zi-xiang Hu1, Qing Xie M.D. 3, Ning Lan Ph.D. 4
1 Laboratory of Neurorehabilitation Engineering, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
2 Laboratory of Neurorehabilitation Engineering, School of Biomedical Engineering; Department of Rehabilitation Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
3 Department of Rehabilitation Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
4 Laboratory of Neurorehabilitation Engineering, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China; Division of Biokinesiology and Physical Therapy, Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA
|Date of Acceptance||05-Nov-2017|
|Date of Web Publication||8-Jan-2018|
Laboratory of Neurorehabilitation Engineering, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China; Division of Biokinesiology and Physical Therapy, Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA
Department of Rehabilitation Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai
Source of Support: This work was supported in part by the National Natural Science Foundation of China, No. 61361160415, 81271684, 81501570; the Major State Basic Research Development of China (973 Program), No. 2011CB013304; the Medicine-Engineering Interdisciplinary Research Grant from Shanghai Jiao Tong University in China, No. YG2014ZD09; and a grant from the Youth Eastern Scholar Program at Shanghai Institutions of Higher Learning in China, No. QD2015007., Conflict of Interest: None
Essential tremor, also referred to as familial tremor, is an autosomal dominant genetic disease and the most common movement disorder. It typically involves a postural and motor tremor of the hands, head or other part of the body. Essential tremor is driven by a central oscillation signal in the brain. However, the corticospinal mechanisms involved in the generation of essential tremor are unclear. Therefore, in this study, we used a neural computational model that includes both monosynaptic and multisynaptic corticospinal pathways interacting with a propriospinal neuronal network. A virtual arm model is driven by the central oscillation signal to simulate tremor activity behavior. Cortical descending commands are classified as alpha or gamma through monosynaptic or multisynaptic corticospinal pathways, which converge respectively on alpha or gamma motoneurons in the spinal cord. Several scenarios are evaluated based on the central oscillation signal passing down to the spinal motoneurons via each descending pathway. The simulated behaviors are compared with clinical essential tremor characteristics to identify the corticospinal pathways responsible for transmitting the central oscillation signal. A propriospinal neuron with strong cortical inhibition performs a gating function in the generation of essential tremor. Our results indicate that the propriospinal neuronal network is essential for relaying the central oscillation signal and the production of essential tremor.
Keywords: nerve regeneration; neurodegeneration; essential tremor; propriospinal neurons; motoneuron; reflex; modeling; motor control; oscillation; neurological disorder; movement disorder; computational neuroscience; neural regeneration
|How to cite this article:|
Qu He, Niu CM, Li S, Hao Mz, Hu Zx, Xie Q, Lan N. Neural computational modeling reveals a major role of corticospinal gating of central oscillations in the generation of essential tremor. Neural Regen Res 2017;12:2035-44
|How to cite this URL:|
Qu He, Niu CM, Li S, Hao Mz, Hu Zx, Xie Q, Lan N. Neural computational modeling reveals a major role of corticospinal gating of central oscillations in the generation of essential tremor. Neural Regen Res [serial online] 2017 [cited 2019 Mar 19];12:2035-44. Available from: http://www.nrronline.org/text.asp?2017/12/12/2035/221161
Hong-en Qu, Chuanxin M. Niu. These authors contributed equally to this paper.
| Introduction|| |
Essential tremor, one of the most common neurological disorders (Louis, 2005; Zhao et al., 2011; Hallett, 2014), is often characterized as postural (Martinelli et al., 1987) and/or kinetic tremors accompanying a motor action. Both the postural and kinetic types of essential tremor typically occur in the forearm and hand with frequencies ranging from 4 to 12 Hz (Deuschl et al., 1998; Brennan et al., 2002). A strong correlation was found between the frequencies on electroencephalogram and limb electromyogram in essential tremor patients (Halliday et al., 2000; Hellwig et al., 2001, 2003; Raethjen et al., 2007; Hellriegel et al., 2012), providing insight into the pathological origin of the disorder. Essential tremor is considered to result from the activity of a single central oscillator that involves the cerebello-thalamo-cortical pathway (Pinto et al., 2003; Raethjen and Deuschl, 2012). However, the efferent pathway of the central oscillation signal (COS), from the brain to the spinal cord, and the neuronal circuits within the spinal cord involved in the generation of essential tremor remain to be identified.
An efferent pathway that could potentially convey oscillation signals for essential tremor is the propriospinal system. The involvement of propriospinal neurons in the neural control of movements has been suggested in previous studies (Isa et al., 2006; Alstermark et al., 2007), possibly indirectly via corticospinal projections. Although the roles of propriospinal neurons in human movement control are unclear, a realistic virtual arm model with authentic spinal neural circuits (Lan and He, 2012; He et al., 2013; Niu et al., 2017) makes it possible to test hypotheses on the role of propriospinal neurons in generating subcortical movement patterns.
A previous computational study (Hao et al., 2013) suggested that Parkinsonian tremor is transmitted from the motor cortex to the peripheral neuromuscular system through the multisynaptic corticospinal pathway, which involves the propriospinal neuronal network (Alstermark et al., 2007). The study revealed that the propriospinal neuronal network plays an important role in generating alternating tremor patterns in antagonistic muscles from central oscillations in Parkinson's disease patients. In clinical practice, essential tremor and Parkinson's disease often cause diagnostic confusion because of overlapping similarities in symptoms (Jain et al., 2006).
In this study, we used a computational model of the corticospinal virtual arm (CS-VA) with propriospinal neurons (Alstermark et al., 2007) and spinal circuitry (Mileusnic et al., 2006; Mileusnic and Loeb, 2006) to investigate the corticospinal mechanisms involved in essential tremor. Several simulation experiments were performed to investigate: (I) the corticospinal efferent pathway related to essential tremor; (II) the role of the propriospinal neuronal network in producing essential tremor; and (III) the effect of peripheral parameters, such as reflex gain, on essential tremor. We also discussed the qualitative differences between our simulation results and data from patients with essential tremor in other clinical studies (Elble et al., 1987; Britton et al., 1994; Elble et al., 1994a, b; Gao, 2004; Heroux et al., 2006; Hellwig et al., 2009; Mostile et al., 2012).
| Materials and Methods|| |
Corticospinal-virtual arm model
In our previous studies (Lan and He, 2012; He et al., 2013, 2015), we developed a computational model that simulates the movement of the human arm, and which includes details of the spinal circuitry thought to contribute to the neural control of movement. This virtual arm model successfully explains the functional significance of alpha-gamma coactivation during arm movements (Li et al., 2014, 2015). Here, we improve the original virtual arm model by adding corticospinal projections. This CS-VA model is a multiscale model which can compute central motor commands into peripheral neural signals and activate lower motor neurons (MNs) in the forelimbs of humans and other mammals. Simultaneously, the proprioceptive afferents inform the brain of the dynamic and kinematic states of the forelimbs and regulate the activation of MNs through spinal reflex circuitry. To implement these functions, the CS-VA model comprises three parts: the propriospinal neuronal network (Alstermark et al., 2007; Hao et al., 2013), spinal reflex circuitry (Mileusnic et al., 2006; Mileusnic and Loeb, 2006), and the virtual arm model (He et al., 2013) [Figure 1].
|Figure 1: Corticospinal virtual arm (CS-VA) model used in this simulation study.|
The CS-VA model developed in this study consists of the corticospinal pathway and the peripheral sensorimotor virtual arm. The four descending pathways are the α monosynaptic pathway from the primary motor cortex directly to the MN pool, the a multisynaptic pathway mediated by the propriospinal neuronal (PN) network, the γ dynamic pathway (γd), which also involves the PN network, and the γ static pathway (γs), which is re-lated to postural control. An action command is added to the multisynaptic pathway to produce a movement. The central oscillation signal (COS) is sent through one of the four descending pathways to help identify the tremor generating mechanism. The sensorimotor virtual arm model, in-cluding the spinal cord circuitry, virtual muscle, proprioceptors (muscle spindle and Golgi tendon organ, GTO) and musculoskeletal dynamics, is validated to capture the realistic properties of the human upper extremity. The virtual arm has two degrees of freedom (DOF) with pairs of antag-onistic muscles—pectoralis major clavicle (PC) and deltoid posterior (DP) for the shoulder, brachialis (BS) and triceps lateral (Tlt) for the elbow, and the biceps short head (Bsh) and triceps long head (Tlh) across both joints.
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As shown in [Figure 1], cortical motor commands are conveyed to α MNs through monosynaptic or multisynaptic pathways. Cortical motor commands are also delivered to γ static (γs, monosynaptic pathway) and γ dynamic (γd, multisynaptic pathway) MNs, which innervate muscle spindles. The movement commands of α dynamic MNs are integrated with the γ dynamic commands at the propriospinal neuronal network. The virtual muscles (Cheng et al., 2000) and spindles (Mileusnic et al., 2006) are activated by the outputs of α and γ MNs, respectively. The dynamic and kinematic movement trajectories are calculated using a biomechanical model of the arm. These trajectories are encoded in the primary and secondary afferents from the muscle spindles and Golgi tendon organs of the recruited muscles. The spinal reflex circuitry in [Figure 2] regulates the outputs of α MNs according to the recurrent inhibition and reflex actions provided by the Ia, Ib and II afferents. The spinal reflex circuitry and virtual arm subsystems are based on real physiological data (Song et al., 2008a, b), and have been verified in previous studies (Song et al., 2008a; He et al., 2013). All the component models were integrated in the SIMULINK\MATLAB (MathWorks, Natick, MA, USA) platform for simulation.
|Figure 2: Model of the propriospinal neuronal (PN) network in the corticospinal pathways of one pair of antagonistic muscles.|
This model is based on experimentally identified PN connections (dashed line) and spinal reflex circuitry. Subscript “d” and “s” for a and . refer to “dynamic” and “static”, respectively. “f” and “e” refer to “flex-or” and “extensor”, respectively. αmulti_f and αmulti_e are a signals from the multisynaptic pathway. αmono_f and αmono_e are a signals from the mono-synaptic pathway. de and df are the inhibition gains of . descending commands to the PN. αf and αe are gains of Ia to PN. Pf and Pe are the PN-related reciprocal gains. Sf and Se are stretch reflex gains. Rf and Re are Ia reciprocal inhibition gains. gf and ge are Renshaw cell gains. bf and be are Golgi tendon organ (GTO) feedback gains. The outputs of GTO and spindles feedback onto the spinal cord, and are integrated with the descending and PN-processed signals to produce activating signals (Uf and Ue) that control the muscles.
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Six muscles were included in the virtual arm model, including three pairs of antagonistic muscles with two degrees of freedom. The pectoralis major (clavicle portion) and deltoid posterior were selected as the flexor and extensor of the shoulder joint, respectively. The brachialis (BS) and triceps lateral (Tlt) were selected for the elbow joint; and the biceps short head (Bsh) and triceps long head (Tlh) were selected as the bi-articular muscles [Figure 1].
Both the physiological and mechanical signals simulated by the CS-VA model were collected and analyzed. The simulated data were compared with experimental data from patients with essential tremor.
We simulated a fast elbow extension movement using the CS-VA model. To focus on the origin of essential tremor, the simulation experiments were simplified into a pair of antagonistic muscles, the biceps and triceps, which controlled the elbow movement. In the simulation, the COS was considered always present. Hence, after the CS-VA was initialized and the virtual arm was in a stable resting state (see [Table 1] for details of model initiation), one of the cortical oscillating commands was used to drive the CS-VA model in different stimulation experiments. After this, a set of pulses were passed to the α multisynaptic pathway in the CS-VA model to drive the model to perform a fast elbow extension, similar to what occurs in the real world. In this movement, the virtual arm changed its elbow angle and maintained this new posture for a while after the movement.
Simulation with different central commands
Four different sources of COS were simulated to identify the one producing the most realistic essential tremor. This experiment was performed because only a few studies found a strong association between the COS and limb electromyogram within the frequency range of essential tremor (Hellwig et al., 2001, 2003; Raethjen et al., 2007). This suggests that the route from COS to muscle activity might be convoluted.
The four sources of COS were the following: α in the monosynaptic pathway (αmono), α in the multisynaptic pathway (αmulti), and γs and γd tracts. By matching the simulation results to the biomechanical features of essential tremor in the clinic, we should be able to identify the neural tract most likely to cause essential tremor.
αmono as the COS: Evidence of direct cortico-motoneuronal connections was found in studies on the voluntary control of the hand (Lemon et al., 1998), and this pathway from the cortex is critical in controlling hand dexterity (Lemon, 2008; Isa et al., 2013). Thus, it was possible that the oscillation in the cortex was delivered through this pathway. The first set of stimulation experiments was performed using αmono as the COS. In this case, the COS directly impinged on the α MN pool [Figure 1], which was further modulated by the spinal reflex involving Ia and Ib afferents, and by recurrent inhibition. The value of αmulti was set at 0. The γs signal, which was placed directly upon the γ MN, was at a constant value. The γd, which was set at a constant bias value of 0.5, was placed into the propriospinal neuronal network and on the γ MN separately. The gains of the spinal reflex were set within a range used in other published studies (Lan and He, 2012; He et al., 2013). In the simulation, a rectified sinusoid was selected to approximate the αmono, at a frequency of 4–7 Hz and an amplitude of 0.1–0.5. The αmono could be described as follows:
where A = 0.1, 0.2, 0.3, 0.4 and 0.5; ω = 4, 5, 6 and 7; and k = 1, 2, 3…
In the simulation experiments, formula 1 was also used for the other COSs.
αmulti as the COS: Two descending pathways connect the brain to the MN pool—the direct and indirect pathways. The direct pathway connects the primary motor cortex to the MN pool, and was simulated in the first simulation experiment. In contrast, the indirect pathway is a multisynaptic pathway connecting the brain to MNs via the propriospinal neural network. The presence of numerous indirect pathways from the brain to MNs has been shown in primates and humans (Alstermark et al., 2007; Riddle et al., 2009; Alstermark et al., 2011). These indirect pathways are mediated by intercalated neurons, such as segmental interneurons, propriospinal neurons and reticulospinal neurons. Propriospinal neurons are involved in the control of fine voluntary movements (Alstermark et al., 2011), as shown in experiments with monkeys. Hence, the COS could be transmitted through this pathway to produce essential tremor. We again chose formula 1 to approximate the COS from αmulti. The COS was modulated in the propriospinal neuronal network [Figure 2] by γd before impinging on the MN pool. The γd values used in the first simulation were also used here. The propriospinal neuronal process could be described as follows:
where Pf and Pe are the propriospinal neuronal outputs to the α MN of the flexor and extensor muscles, respectively; αmulti is the α signal in the multisynaptic pathway; ds and df are the inhibition of the gains of γd; ae and af are the feedback gains of the Ia afferent onto the propriospinal neurons; and υe and υf are proportional to Ia and are afferent discharge frequencies of the spindles from the flexor and extensor muscles, respectively. The subscripts f and e are variables for the flexor and extensor muscles, respectively. This formula was obtained from a previous study (Hao et al., 2013) and was modified based on physiological evidence in other studies (Zehr et al., 2004; Zehr and Hundza, 2005; Alstermark et al., 2007; Alstermark and Isa, 2012). This model highlights the importance of spinal circuits in the modulation of muscle activity patterns.
γs as the COS: Both α and γ commands are known to be involved in normal voluntary movement. Based on the correlation of the behavior of these commands during midbrain stimulation with changes in the muscle spindle afferent responses to muscle stretching, the cortical γ commands were classified into two types—γ static (γs) and γ dynamic (γd) (Taylor et al., 2000). Some researchers consider γs to be the temporal template of the intended movement and afferent biasing through bag intrafusal fibers (Ellaway et al., 2002). According to this concept, if the COS was added to the γs, a tremor movement might occur. In the third simulation experiment, we used γs (the COS) to drive the model. This signal was directly passed onto the γ MN, similar to formula 1. In the CS-VA model, this signal could affect spindle feedback, which is the input to the α MN pool. Therefore, when the subject wants to move, tremor might result.
γ dynamic as the COS: According to previous studies (Taylor et al., 2000, 2004, 2006; Alstermark et al., 2007), γ dynamic activity is related to movement control during locomotion in decerebrated cats. These studies show that γd firing frequency increases unexpectedly from zero to maximum during the onset of muscle shortening, and then continues into the beginning of lengthening (Taylor et al., 2000). This observation suggests that γd serves an important function in movement control. Thus, γ dynamic commands might be associated with the COS at the essential tremor frequency. In the CS-VS model, similar to the second simulation, the γd was entered into the propriospinal neuronal network and the γ MN, but the value was a combination of the bias value of 0.5 and the rectified sinusoidal wave described in formula 1. According to formula 2, the inhibition is not a constant value, but rather a fluctuating value. If the movement command is entered into propriospinal neurons, the abnormal γd can modulate the movement command in an oscillating way, affecting the α MN pool indirectly. In addition, the COS was entered into the γ MN to adjust the spindle output, which is also related to the MN pool. Hence, this might be another cause of tremor.
Simulation with different stretch reflex gains
We also examined factors that could potentially affect the amplitude and frequency of essential tremor. Based on data from essential tremor patients (Elble et al., 1994b; Matsumoto et al., 1999; Gao, 2004; Mostile et al., 2012), we proposed the following: (I) The frequency of essential tremor is only determined by the COS, as shown by corticomuscular coherence studies (Hellwig et al., 2001; Raethjen et al., 2007); and (II) The amplitude of essential tremor is related to two components, the COS and the mechanical reflex (Elble et al., 1987).
Previous studies have examined the contribution of the mechanical reflex to essential tremor by varying the inertial loading. However, obtaining accurate reflex gain values in human movements is difficult, and adjusting these values to determine a relationship between the amplitude of essential tremor and the stretch reflex is even more difficult. The main reflexes in our CS-VA model can be conveniently adjusted to determine biomechanical changes. Hence, in another set of simulations, stretch reflex gains were adjusted from 0.1 to 0.5. For each stretch reflex gain value, both the amplitude and frequency of the COS were adjusted. From the biomechanical data obtained with our model, we could quantitatively analyze the relationship between the stretch gain and the amplitude of the essential tremor.
| Results|| |
Tremor generated by the COS
With the hypothesis that the central oscillation is the originating cause of essential tremor, the abnormal joint angles and muscle behavior were calculated from the CS-VA model (plotted in [Figure 3], [Figure 4], [Figure 5], [Figure 6], and the spectra were analyzed. In these figures, all COSs were set at a frequency of 5 Hz and an amplitude of 0.3.
|Figure 3: Features of movement and muscle activity in the simulation experiment (αmono).The figure illustrates the results of the experiment in which the cen-tral oscillation signal is the αmono. The frequency of αmono is 5 Hz and the amplitude is 0.3. In our design, an action occurs at 15 seconds. (A) Elbow angle traces before and af-ter the action; (B) spectrum of this trace before and after the action; (C) the velocity of the elbow trace; (D) the spectrum of this velocity; (E) the muscle activity of the bi-ceps and triceps before and after the movement; (F) the spectrum of the pair of antagonistic muscles before and after the action. S: Sec-ond.|
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|Figure 4: Features of movement and muscle activity in the simulation experiment (αmulti).|
The figure illustrates the results of an experiment in which the central oscillation signal is the αmulti. The frequency of αmulti is 5 Hz and the amplitude is 0.3. In our design, an action occurs at 15 seconds. (A) Elbow angle traces before and after the action; (B) the spectrum of this trace before and after the action; (C) the velocity of the elbow trace; (D) the spectrum of this velocity; (E) the muscle activity of the biceps and triceps before and after the movement; (F) the spectrum of the pair of antag-onistic muscles before and after the action. S: Second.
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|Figure 5: Features of movement and muscle activity in the simulation experiment (γs).The figure illustrates the results of an experiment in which the central oscillation signal is the γs. The frequency of γs is 5 Hz and the amplitude is 0.3. In our design, an action occurs at 15 seconds. (A) Elbow angle traces before and after the action; (B) the spectrum of this trace before and after the action; (C) the velocity of the elbow trace; (D) the spectrum of this velocity; (E) the muscle activ-ity of the biceps and triceps before and after the movement; (F) the spectrum of the pair of antago-nistic muscles before and after the action. |
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|Figure 6: Features of movement and muscle activity in the simulation experiment (γd).|
The figure illustrates the results of an experiment in which the central oscillation signal is the γd. The frequency of γd is 5 Hz and the amplitude is 0.3. In our design, an action occurs at 15 seconds. (A) Elbow angle traces before and after the action; (B) the spectrum of this trace before and after the action; (C) the velocity of the elbow trace; (D) the spectrum of this velocity; (E) the muscle activ-ity of the biceps and triceps before and after the movement; (F) the spectrum of the pair of antago-nistic muscles before and after the action.
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In the first experiment, αmono was set as the COS pathway. Under this condition, the elbow angle always oscillated before and after the action [Figure 3]A, [Figure 3]C. The tremor amplitude was approximately 0.3 degree, and the amplitude decreased after the action occurred, according to the movement and velocity spectra [Figure 3]B, [Figure 3]D. The biceps and triceps were activated synchronously by this type of COS [Figure 3]E, and the two muscles had nearly the same amplitude of activity during co-contraction, according to the spectral analysis [Figure 3]F, [Figure 3]G. The movement and velocity spectra of the elbow movement before and after the action did not show any obvious difference, contradicting the widely-held concept that essential tremor is an action tremor.
In the second experiment, αmulti was taken as the COS pathway. As shown in [Figure 4]A, the elbow angle did not change at the resting state, and muscle activity showed no corresponding changes [Figure 4]B. A tremor occurred after the elbow extension. The tremor amplitude was very small, but the spectral analysis of the movement revealed the existence of two peaks, namely, one at 5 Hz and another at 1 Hz, which may have caused confusion in the observation of the tremor. However, the spectral analysis showed an obvious single peak at the tremor frequency (5 Hz) in elbow velocity after the extension movement [Figure 4]D. The maximum value of the spectrum at 5 Hz was over 0.75, reflecting the oscillating movement. After the action, the muscle activity was in a co-contraction firing pattern of antagonistic muscles, caused by the α COS in the multisynaptic pathway. This firing pattern caused elbow oscillation, but the tremor amplitude was very small. The spectrum of the muscle activity demonstrated the co-contraction pattern at 5 Hz. The kinetic features before and after the elbow action agreed with some characteristics of essential tremor in clinical studies, and the muscle activity had a co-contraction pattern similar to the electromyogram in essential tremor patients.
In the third experiment, γs was taken as the initial cause of essential tremor. The commands for postural control showed an abnormal firing pattern and reached the γ MNs directly. The elbow angle showed very small tremor after the movement [Figure 5]A. Muscle activity was enhanced after the action [Figure 5]E, but the spectral analyses of the two muscles showed that muscle activity was neither synchronous nor alternating. In addition, the muscle activity spectra showed that there was no difference in tremor frequency, whether the arm moved or not [Figure 5]F, [Figure 5]G.
The last experiment used hd as the COS. Before it reached the γ MN, d was conveyed through the propriospinal neuronal network. Similar to the second experiment, no tremor was found at resting state, and the tremor behavior was observed after the action [Figure 6]A. Compared with the second experiment, the tremor in the elbow trace in [Figure 6]A was easily detectable because tremor amplitude was relatively high. The elbow angle oscillated between 76° and 77° at a frequency of 5 Hz, the same as the COS frequency. The biggest difference between the simulation experiments was that muscle activity showed an alternating pattern in the present simulation [Figure 6]E. The spectra showed that muscle activity peaked at 5 Hz, the tremor frequency, in both the biceps and triceps, despite different activity levels [Figure 6]F, [Figure 6]G.
Influence of the oscillating parameters of COS on the simulated tremor
Our current simulation experiments indicate that αmulti and αd are the COSs underlying essential tremor. We also examined the COS parameters that influence the amplitude of elbow oscillation, i.e. the amplitude and frequency. [Figure 7] shows the relationships between these COS parameters and the elbow oscillating amplitude. The amplitude of umulti and nd ranged from 0.1 to 0.5. The frequency of rmulti and αd was adjusted from 4 to 7 Hz. Regardless of the COS (whether αmulti or od), the same regulatory effect was found—frequency had a greater impact on tremor amplitude. The lower the COS frequency, the larger the tremor amplitude. When the frequency was reduced from 4 to 5 Hz, the tremor amplitude decreased drastically. However, the tremor amplitude changed less in the range between 5 and 6 Hz. Moreover, the tremor disappeared when COS in the tmulti pathway was at 7 Hz. As the COS was delivered through the αmulti pathway, the tremor amplitude first increased along with the increase in COS amplitude from 0.1 to 0.4, and reached the maximum value when the COS amplitude was 0.4, and then decreased as COS amplitude increased from 0.4 to 0.5 [Figure 7]A. The tremor amplitude monotonously increased when COS was wd, as shown in [Figure 7]B. These findings show that whether the COS is from the αmulti or hd pathway, the tremor frequency is determined by the frequency of the COS. COS amplitude only influences tremor amplitude.
|Figure 7: Influence of the frequency and amplitude of the central oscillation signal (COS) on tremor amplitude.|
(A) The tremor amplitude varies with frequency and the amplitude of the COS, when the COS is αmulti. (B) The tremor amplitude varies with frequency and amplitude of the COS, when the COS is γd.
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Stretch reflex-caused oscillation contributed to essential tremor
[Figure 8] shows the effect of stretch reflex gain on the amplitude. Given that the COS was from the tmulti pathway, with an amplitude of 0.3, the tremor amplitude was only affected by the COS frequency. Increasing stretch reflex gain did not influence tremor amplitude, as shown in [Figure 8]A. When the COS frequency was fixed at 5 Hz, and the COS amplitude was within 0.3, increasing stretch reflex gain did not affect tremor amplitude, as shown in [Figure 8]B. However, increasing stretch reflex gain reduced tremor amplitude when the COS amplitudes were 0.4 and 0.5 [Figure 8]B. As shown in [Figure 8]C, except when the d was at 4 Hz (the wd amplitude was maintained at 0.3), increasing stretch reflex gain weakly affected tremor amplitude, compared with the effect of td frequency. The amplitude of fd and the stretch reflex gain had similar effects on the tremor amplitude [Figure 8]D.
|Figure 8: Relationship between tremor amplitude and reflex gain.|
(A, B) Relationship between tremor amplitude and reflex gain when the central oscillation signal (COS) is amulti. (A) Changes in tremor am-plitude when the stretch reflex gain varies from 0.1 to 0.5 at different tremor frequencies (4, 5 and 6 Hz) and the same COS amplitude of 0.3. (B) Changes in tremor amplitude when stretch reflex gain varies from 0.1 to 0.5 at different COS amplitudes and the same frequency of 5 Hz. (C, D) The relationship between tremor amplitude and reflex gain when the COS is γd. (C) Changes in tremor amplitude when stretch reflex gain varies from 0.1 to 0.5 at different tremor frequen-cies (4, 5, 6 and 7 Hz) and the same COS amplitude of 0.3. (D) Changes in tremor amplitude when stretch reflex gain varies from 0.1 to 0.5 at different COS amplitudes and the same frequency of 5 Hz.
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| Discussion|| |
In this study, a set of simulation experiments were performed to identify the efferent spinal pathway conveying the COS from the brain, and whether the propriospinal neuronal network is involved in the generation of essential tremor. In our simulation experiments, nmono and αs were excluded as the COS pathways causing essential tremor, because the kinetic and physiological characteristics did not match the clinical features of essential tremor.
Essential tremor can be classified into two types, based on clinical assessment: type A and type B (Deuschl et al., 1987; Milanov, 2001). Deuschl et al. showed that in type A essential tremor, electromyography shows synchronous activity in the antagonistic muscles, whereas electromyography shows alternating activity in the antagonists in type B essential tremor. Moreover, in Milanov's study, more than 200 essential tremor patients were recruited, and similar results were obtained. Correlating the clinical characteristics with the simulation results suggests that the tremor caused by ymulti is most likely the type A variety. In the second experiment, the tremor caused by COS from the αmulti pathway did not appear in the resting state and had low amplitude after elbow extension. These kinetic features are similar to the clinical characteristics. In addition, the muscle activity was of a co-contraction pattern. The tremor caused by the γd pathway COS in the simulation experiments can be identified as type B using the same method. The kinetic characteristics of the elbow and muscle activities matched the signs of type B essential tremor, with the tremor amplitude larger than in type A, with alternating muscle activity (Britton et al., 1994).
Both types of COS were modulated by the propriospinal neuronal network in the spinal cord. This shows the importance of the propriospinal neuronal network in controlling human movement. The propriospinal neuronal network, which serves a gating function, modulated the central commands from the brain to the spinal cord. Our findings could be related to observations of the corticospinal tract in patients with ET and PD (Lu et al., 2016). In terms of movement control angle, essential tremor can be identified clearly at the spinal cord level. In type A essential tremor, propriospinal neurons gate the αmulti COS. Indeed, essential tremor patients show no tremor in the resting state. However, when COS was added to the movement control signals, it surmounted the gating by the propriospinal neurons to reach the α MNs. This explains the occurrence of tremors when an action is performed. In type B essential tremor, the γd COS overcame the propriospinal neuronal gating function by lowering the gating value. At resting state, α is too small to cause tremor, even when the propriospinal neuronal gating function is impaired. When a patient wants to move or maintain a posture, and α is sufficiently large, propriospinal neuronal gating would be unable to prevent tremor.
In previous studies, we used a similar model that included the propriospinal neuronal network to simulate Parkinson's disease tremors. The simulation results matched the clinical features of Parkinson's disease tremors. Thus, the propriospinal neuronal network is involved in both tremors. Essential tremor and Parkinson's disease tremors share the same circuits in the spinal cord. Indeed, the signs of both tremors are similar and overlapping, and distinguishing them is clinically difficult.
In a previous study (Elble et al., 1987), the stretch reflex in essential tremor was examined, and the tremor was found to be influenced by two components—COS and the mechanical reflex. However, tremor frequency was determined by the central oscillation. Our current results are in agreement with these observations. COS frequency not only impacted tremor frequency, but also strongly influenced tremor amplitude in our simulation experiments, regardless of the COS pathway (fmulti or rd) [Figure 7] and [Figure 8]. An advantage of the CS-VA model, the influence of the stretch reflex gain can be directly investigated in the simulation. In type A essential tremor, the stretch reflex gain had none or little effect on the tremor amplitude, as shown in [Figure 8]. In type B essential tremor, increasing stretch reflex gain aggravated the tremor amplitude until the tremor amplitude was over 0.035. Our results provide novel insight into the neurological basis of essential tremor.
In our study, essential tremor was elicited by the COS through the multisynaptic corticospinal pathway involving the propriospinal neuronal network. The propriospinal neuron plays an important role in gating the COS at resting state, preventing the COS from passing down to the peripheral neuromuscular system. During the performance of an active task, cortical inhibition of the propriospinal neuron is reduced or removed by task commands, and the COS can be transmitted to the peripheral neuromuscular system freely, causing a tremor activity that accompanies the task. The simulation analyses here suggest that the propriospinal neuronal network plays a role in the gating of the cortical oscillation signal, preventing it from reaching the peripheral muscles in the resting state, but allowing it to pass to the muscles during an active task.
In conclusion, COS frequency is the major factor influencing tremor amplitude, with a greater impact than COS amplitude or stretch reflex gain. Computational modeling is an effective method of simulating essential tremor and identifying abnormal neural networks.
Acknowledgments: The authors thank Drs. Yong Bao and Li-jun Cui for their help during the discussion.
Author contributions: HEQ constructed the simulation, collected the data, analyzed the results, and participated in the production of the manuscript. CMN contributed to the design of the experiment, analyzed the results, and produced part of the manuscript. SL, MZH and ZXH contributed to the modeling, simulation and data collection. QX contributed the design and analysis of the study. NL provided the original concept and design of the study, analyzed the results, and jointly produced the manuscript. All authors approved the final version of the paper.
Conflicts of interest: None declared.
Data sharing statement: Datasets analyzed during the current study are available from the corresponding author on reasonable request.
Plagiarism check: Checked twice by iThenticate.
Peer review: Externally peer reviewed.
Copyedited by Patel B, Frenchman B, Wang J, Li CH, Qiu Y, Song LP, Zhao M
| References|| |
Alstermark B, Isa T (2012) Circuits for skilled reaching and grasping. Annu Rev Neurosci 35:559-578.
Alstermark B, Isa T, Pettersson LG, Sasaki S (2007) The C3-C4 propriospinal system in the cat and monkey: a spinal pre-motoneuronal centre for voluntary motor control. Acta Physiol 189:123-140.
Alstermark B, Pettersson LG, Nishimura Y, Yoshino-Saito K, Tsuboi F, Takahashi M, Isa T (2011) Motor command for precision grip in the macaque monkey can be mediated by spinal interneurons. J Neurophysiol 106:122-126.
Brennan KC, Jurewicz EC, Ford B, Pullman SL, Louis ED (2002) Is essential tremor predominantly a kinetic or a postural tremor? A clinical and electrophysiological study. Mov Disord 17:313-316.
Britton TC, Thompson PD, Day BL, Rothwell JC, Findley LJ, Marsden CD (1994) Rapid wrist movements in patients with essential tremor. The critical role of the second agonist burst. Brain 117(Pt 1) :39-47.
Cheng EJ, Brown IE, Loeb GE (2000) Virtual muscle: a computational approach to understanding the effects of muscle properties on motor control. J Neurosci Methods 101:117-130.
Deuschl G, Bain P, Brin M (1998) Consensus statement of the Movement Disorder Society on Tremor. Ad Hoc Scientific Committee. Mov Disord 13 Suppl 3:2-23.
Deuschl G, Lucking CH, Schenck E (1987) Essential tremor: electrophysiological and pharmacological evidence for a subdivision. J Neurol Neurosurg Psychiatry 50:1435-1441.
Elble RJ, Higgins C, Hughes L (1994a) Essential tremor entrains rapid voluntary movements. Exp Neurol 126:138-143.
Elble RJ, Higgins C, Leffler K, Hughes L (1994b) Factors influencing the amplitude and frequency of essential tremor. Mov Disord 9:589-596.
Elble RJ, Higgins C, Moody CJ (1987) Stretch reflex oscillations and essential tremor. J Neurol Neurosurg Psychiatry 50:691-698.
Ellaway P, Taylor A, Durbaba R, Rawlinson S (2002) Role of the fusimotor system in locomotion. Sensorimotor Control Mov Posture 508:335-342.
Gao JB (2004) Analysis of amplitude and frequency variations of essential and Parkinsonian tremors. Med Biol Eng Comput 42:345-349.
Hallett M (2014) Tremor: pathophysiology. Park Relat Disord 20 Suppl 1:S118-22.
Halliday DM, Conway BA, Farmer SF, Shahani U, Russell AJ, Rosenberg JR (2000) Coherence between low-frequency activation of the motor cortex and tremor in patients with essential tremor. Lancet 355:1149-1153.
Hao M, He X, Xiao Q, Alstermark B, Lan N (2013) Corticomuscular transmission of tremor signals by propriospinal neurons in Parkinson's disease. PLoS One 8:e79829.
He X, Du YF, Lan N (2013) Evaluation of feedforward and feedback contributions to hand stiffness and variability in multijoint arm control. IEEE Trans Neural Syst Rehabil Eng 21:634-647.
He X, Hao M-Z, Wei M, Xiao Q, Lan N (2015) Contribution of inter-muscular synchronization in the modulation of tremor intensity in Parkinson's disease. J Neuroeng Rehabil 12:1.
Hellriegel H, Schulz EM, Siebner HR, Deuschl G, Raethjen JH (2012) Continuous theta-burst stimulation of the primary motor cortex in essential tremor. Clin Neurophysiol 123:1010-1015.
Hellwig B, Haussler S, Schelter B, Lauk M, Guschlbauer B, Timmer J, Lucking CH (2001) Tremor-correlated cortical activity in essential tremor. Lancet 357:519-523.
Hellwig B, Mund P, Schelter B, Guschlbauer B, Timmer J, Lucking CH (2009) A longitudinal study of tremor frequencies in Parkinson's disease and essential tremor. Clin Neurophysiol 120:431-435.
Hellwig B, Schelter B, Guschlbauer B, Timmer J, Lucking CH (2003) Dynamic synchronisation of central oscillators in essential tremor. Clin Neurophysiol 114:1462-1467.
Heroux ME, Parisi SL, Larocerie-Salgado J, Norman KE (2006) Upper-extremity disability in essential tremor. Arch Phys Med Rehabil 87:661-670.
Isa T, Kinoshita M, Nishimura Y (2013) Role of direct vs. indirect pathways from the motor cortex to spinal motoneurons in the control of hand dexterity. Front Neurol 4:191.
Isa T, Ohki Y, Seki K, Alstermark B (2006) Properties of propriospinal neurons in the C3-C4 segments mediating disynaptic pyramidal excitation to forelimb motoneurons in the macaque monkey. J Neurophysiol 95:3674-3685.
Jain S, Lo SE, Louis ED (2006) Common misdiagnosis of a common neurological disorder: how are we misdiagnosing essential tremor? Arch Neurol 63:1100-1104.
Lan N, He X (2012) Fusimotor control of spindle sensitivity regulates central and peripheral coding of joint angles. Front Comput Neurosci 6:66.
Lemon RN (2008) Descending pathways in motor control. Annu Rev Neurosci 31:195-218.
Lemon RN, Baker SN, Davis JA, Kirkwood PA, Maier MA, Yang HS (1998) The importance of the cortico-motoneuronal system for control of grasp. Novartis Found Symp 218:202-215; discussion 215-218.
Li S, He X, Lan N (2014) Modular control of movement and posture by the corticospinal alpha-gamma motor systems. In, pp 4079-4082. IEEE.
Li S, Zhuang C, Hao M, He X, Marquez JC, Niu CM, Lan N (2015) Coordinated alpha and gamma control of muscles and spindles in movement and posture. Front Comput Neurosci 9:122.
Louis ED (2005) Essential tremor. Lancet Neurol 4:100-110.
Lu MK, Chen CM, Duann JR, Ziemann U, Chen JC, Chiou SM, Tsai C-H (2016) Investigation of motor cortical plasticity and corticospinal tract diffusion tensor imaging in patients with Parkinsons disease and essential tremor. PLoS One 11:e0162265.
Martinelli P, Gabellini AS, Gulli MR, Lugaresi E (1987) Different clinical features of essential tremor: a 200-patient study. Acta Neurol Scand 75:106-111.
Matsumoto JY, Dodick DW, Stevens LN, Newman RC, Caskey PE, Fjerstad W (1999) Three-dimensional measurement of essential tremor. Mov Disord 14:288-294.
Milanov I (2001) Electromyographic differentiation of tremors. Clin Neurophysiol 112:1626-1632.
Mileusnic MP, Brown IE, Lan N, Loeb GE (2006) Mathematical models of proprioceptors. I. Control and transduction in the muscle spindle. J Neurophysiol 96:1772-1788.
Mileusnic MP, Loeb GE (2006) Mathematical models of proprioceptors. II. Structure and function of the Golgi tendon organ. J Neurophysiol 96:1789-1802.
Mostile G, Fekete R, Giuffrida JP, Yaltho T, Davidson A, Nicoletti A, Zappia M, Jankovic J (2012) Amplitude fluctuations in essential tremor. Park Relat Disord 18:859-863.
Niu CM, Jalaleddini K, Sohn WJ, Rocamora J, Sanger TD, Valero-Cuevas FJ (2017) Neuromorphic meets neuromechanics, part I: the methodology and implementation. J Neural Eng 14:025001.
Pinto AD, Lang AE, Chen R (2003) The cerebellothalamocortical pathway in essential tremor. Neurology 60:1985-1987.
Raethjen J, Deuschl G (2012) The oscillating central network of Essential tremor. Clin Neurophysiol 123:61-64.
Raethjen J, Govindan RB, Kopper F, Muthuraman M, Deuschl G (2007) Cortical involvement in the generation of essential tremor. J Neurophysiol 97:3219-3228.
Riddle CN, Edgley SA, Baker SN (2009) Direct and indirect connections with upper limb motoneurons from the primate reticulospinal tract. J Neurosci 29:4993-4999.
Song D, Lan N, Loeb GE, Gordon J (2008a) Model-based sensorimotor integration for multi-joint control: development of a virtual arm model. Ann Biomed Eng 36:1033-1048.
Song D, Raphael G, Lan N, Loeb GE (2008b) Computationally efficient models of neuromuscular recruitment and mechanics. J Neural Eng 5:175-184.
Taylor A, Durbaba R, Ellaway PH (2004) Direct and indirect assessment of gamma-motor firing patterns. Can J Physiol Pharmacol 82:793-802.
Taylor A, Durbaba R, Ellaway PH, Rawlinson S (2006) Static and dynamic gamma-motor output to ankle flexor muscles during locomotion in the decerebrate cat. J Physiol 571:711-723.
Taylor A, Ellaway PH, Durbaba R, Rawlinson S (2000) Distinctive patterns of static and dynamic gamma motor activity during locomotion in the decerebrate cat. J Physiol 529 Pt 3:825-836.
Zehr EP, Carroll TJ, Chua R, Collins DF, Frigon A, Haridas C, Hundza SR, Thompson AK (2004) Possible contributions of CPG activity to the control of rhythmic human arm movement. Can J Physiol Pharmacol 82:556-568.
Zehr EP, Hundza SR (2005) Forward and backward arm cycling are regulated by equivalent neural mechanisms. J Neurophysiol 93:633-640.
Zhao J, Yang B, Li JQ, Yan JH, Zhu YH (2011) Application of shifted band-limited multiple fourier linear combiner in pre-estimation and filtering of human tremor. Zhongguo Zuzhi Gongcheng Yanjiu 15:4837-4840.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8]