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  Indian J Med Microbiol
 

Figure 1: Cell culture model to study the intrinsic axonal regeneration response. (A) A three-step set-up to investigate axonal regeneration in cultured neurons. In this example, primary cortical neurons were plated on a glass-bottomed imaging dish and were transfected with various DNA plasmids. The axons of the transfected neurons underwent laser axotomy and were video recorded for 14 hours. The arrow shows the site of injury. (B) Representative images of a non-regenerating axon (upper panels) and a regenerating axon (lower panels) from cortical neurons that were cultured for two weeks. The asterisk indicates the location of the proximal stump after one-hour post-axotomy, while the arrow marks the growth cone at indicated time-point. There is no difference in distal degeneration rate when comparing between regenerating and non-regenerating axons. Scale bars: 50 μm.

Figure 1: Cell culture model to study the intrinsic axonal regeneration response.
(A) A three-step set-up to investigate axonal regeneration in cultured neurons. In this example, primary cortical neurons were plated on a glass-bottomed imaging dish and were transfected with various DNA plasmids. The axons of the transfected neurons underwent laser axotomy and were video recorded for 14 hours. The arrow shows the site of injury. (B) Representative images of a non-regenerating axon (upper panels) and a regenerating axon (lower panels) from cortical neurons that were cultured for two weeks. The asterisk indicates the location of the proximal stump after one-hour post-axotomy, while the arrow marks the growth cone at indicated time-point. There is no difference in distal degeneration rate when comparing between regenerating and non-regenerating axons. Scale bars: 50 μm.