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RESEARCH AND REPORT: STEM CELLS AND NEURAL REGENERATION
Year : 2012  |  Volume : 7  |  Issue : 22  |  Page : 1688-1694

Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro


1 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region; Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China
2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China
3 Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China

Correspondence Address:
Dongsheng Li
Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province
China
Kehuan Lu
State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region
China
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Source of Support: NThis study was supported by the National Natural Science Foundation of China, No. 81070614; the Key Project of the Natural Science Foundation of Hubei Province of China, No. 2008CDA044; and the Natural Science Foundation of Hubei University of Medicine, No. 2011QDZR-2., Conflict of Interest: None


DOI: 10.3969/j.issn.1673-5374.2012.22.002

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In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells. Research Highlights
  1. During the process of neural stem/progenitor cell differentiation, fibroblast growth factor or Sonic Hedgehog proteins or factors may be secreted from other neural cells, and together they compose a niche environment for neurosphere co-culture.
  2. The experimental results were analyzed by reverse transcription-PCR, western blot and enzyme-linked immunosorbent assay, to provide a detailed analysis of mRNA and protein expression.
  3. There were differences between the cell mRNA, protein content and protein secretion levels.
Abbreviations ELISA, enzyme-linked immunosorbent assay


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