• Users Online: 708
  • Home
  • Print this page
  • Email this page
RESEARCH AND REPORT: STEM CELLS AND NEURAL REGENERATION
Year : 2012  |  Volume : 7  |  Issue : 22  |  Page : 1695-1702

Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse


1 Department of Neurology, First Affiliated Hospital, Sun Yat-sen University; Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China
2 Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China
3 Department of Neurology, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China
4 Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
5 Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou 510085, Guangdong Province, China
6 Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China

Correspondence Address:
Cheng Zhang
Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province
China
Login to access the Email id

Source of Support: This work was supported by the National Natural Science Foundation of China, No. 30370510, 30170337, 30400322, 30870851; CMB Fund, No. 4209347; Key Project of the State Ministry of Public Health, No. 2001321; Fok Ying Tung Education Foundation, No. 91029; and Key Projects in the National Science and Technology Pillar Program During the Eleventh Five-Year Plan Period, No. 2006BAI05A07., Conflict of Interest: None


DOI: 10.3969/j.issn.1673-5374.2012.22.003

Rights and Permissions

In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse. Research Highlights
  1. Recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse.
  2. Dystrophin gene and dystrophin-related proteins contribute to induce adipose-derived stem cell differentiation into myoblasts, in a broader attempt to promote autologous stem cell transplantation for Duchenne muscular dystrophy.
Abbreviations microdys, microdystrophin; β-cat, beta-catenin; dko, dystrophin-utrophin double knock-out; MyoD, myogenic differentiation antigen; MHC, myosin heavy chain; MSCs, mesenchymal stem cells


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed825    
    Printed16    
    Emailed0    
    PDF Downloaded91    
    Comments [Add]    

Recommend this journal