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NEUROGENESIS AND NEURAL PLASTICICTY
Year : 2012  |  Volume : 7  |  Issue : 22  |  Page : 1709-1714

Identification of α7 nicotinic acetylcholine receptor on hippocampal astrocytes cultured in vitro and its role on inflammatory mediator secretion


1 Department of Pharmacy, Zhujiang Hospital, Southern Medical University; Department of Pharmacy, the 458 Hospital of Chinese PLA, Guangzhou 510602, Guangdong Province, China
2 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
3 Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510602, Guangdong Province, China

Correspondence Address:
Yong Wang
Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guang-zhou 510282, Guangdong Province
China
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Source of Support: This study was supported by the National Natural Science Foundation of China, No. 30471928 and No. 30973162 and the Natural Science Foundation of Guangdong Province, No. 07005203., Conflict of Interest: None


DOI: 10.3969/j.issn.1673-5374.2012.22.005

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The present study found expressions of α7 nicotinic acetylcholine receptor on hippocampal slices and hippocampal astrocytes using double immunofluorescence stainings. Expression of glial fibrillary acidic protein in the cultured hippocampal slices and hippocampal astrocytes significantly increased, and levels of macrophage inflammatory protein 1α, RANTES, interleukin-1β, interleukin-6, and tumor necrosis factor-α increased in the supernatant of cultured astrocytes following exposure to 200 nM amyloid β protein 1-42. Preconditioning of 10 μM nicotine, a nicotinic acetylcholine receptor agonist, could attenuate the influence of amyloid β protein 1-42 in inflammatory mediator secretion of cultured astrocytes. Experimental findings indicated that α7 nicotinic acetylcholine receptor was expressed on the surface of hippocampal astrocytes, and activated α7 nicotinic acetylcholine receptor was shown to inhibit inflammation induced by amyloid β protein 1-42. Research Highlights
  1. The present study detected the expression of α7 nicotinic acetylcholine receptor on the surface of hippocampal astrocytes.
  2. Activated α7 nicotinic acetylcholine receptor was shown to inhibit inflammation induced by amyloid β protein 1-42.
Abbreviations AD, Alzheimer's disease; Aβ1-42, amyloid β protein 1-42; GFAP, glial fibrillary acidic protein


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