• Users Online: 637
  • Home
  • Print this page
  • Email this page
RESEARCH AND REPORT
Year : 2014  |  Volume : 9  |  Issue : 10  |  Page : 1031-1040

Differential expression of microRNAs in dorsal root ganglia after sciatic nerve injury


1 Department of Orthopedics, the Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China
2 Neurosciences Research Department, Hainan Medical University, Haikou, Hainan Province, China

Correspondence Address:
M.D. Xinan Yi
Neurosciences Research Department, Hainan Medical University, Haikou, Hainan Province
China
Login to access the Email id

Source of Support: This work was supported by the National Natural Science Foundation of China, No. 81160158 and 30860290., Conflict of Interest: None


DOI: 10.4103/1673-5374.133164

Rights and Permissions

This study investigated the possible involvement of microRNAs in the regulation of genes that participate in peripheral neural regeneration. A microRNA microarray analysis was conducted and 23 microRNAs were identified whose expression was significantly changed in rat dorsal root ganglia after sciatic nerve transection. The expression of one of the downregulated microRNAs, microRNA-214, was validated using quantitative reverse transcriptase-PCR. MicroRNA-214 was predicted to target the 3′-untranslated region of Slit-Robo GTPase-activating protein 3. In situ hybridization verified that microRNA-214 was located in the cytoplasm of dorsal root ganglia primary neurons and was downregulated following sciatic nerve transection. Moreover, a combination of in situ hybridization and immunohistochemistry revealed that microRNA-214 and Slit-Robo GTPase-activating protein 3 were co-localized in dorsal root ganglion primary neurons. Western blot analysis suggested that Slit-Robo GTPase-activating protein 3 was upregulated in dorsal root ganglion neurons after sciatic nerve transection. These data demonstrate that microRNA-214 is located and differentially expressed in dorsal root ganglion primary neurons and may participate in regulating the gene expression of Slit-Robo GTPase-activating protein 3 after sciatic nerve transection.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2337    
    Printed29    
    Emailed0    
    PDF Downloaded370    
    Comments [Add]    
    Cited by others 5    

Recommend this journal