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TECHNICAL UPDATES
Year : 2014  |  Volume : 9  |  Issue : 10  |  Page : 1063-1067

Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro


1 Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
2 Operating Room, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China

Correspondence Address:
Qian Dong
Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province
China
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Source of Support: This study was supported by the National Natural Science Foundation of China, No. 81272986 and the Natural Science Foundation of Shandong Province, No. ZR2011HZ002., Conflict of Interest: None


DOI: 10.4103/1673-5374.133172

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This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was significantly suppressed in transfected cells by all three sequence-specific siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was significantly decreased following transfection with CXCR4-specific siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.


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