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RESEARCH ARTICLE
Year : 2015  |  Volume : 10  |  Issue : 3  |  Page : 404-411

Visual bone marrow mesenchymal stem cell transplantation in the repair of spinal cord injury


1 Department of Radiology, First Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China ; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA
2 Department of Radiology, Shanxi Provincial People's Hospital, Taiyuan, Shanxi Province, China
3 Department of Radiology, First Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China
4 Department of Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi Province, China

Correspondence Address:
Jun Xie
Department of Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi Province, China

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Source of Support: This research was supported by the National Natural Science Foundation of China, No. 81371628 and the Postdoctoral Science Foundation of China, No. 2014T70233, 2013M541206 and the Innovation Foundation of Shanxi Medical University First Hospital of China., Conflict of Interest: None


DOI: 10.4103/1673-5374.153688

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An important factor in improving functional recovery from spinal cord injury using stem cells is maximizing the number of transplanted cells at the lesion site. Here, we established a contusion model of spinal cord injury by dropping a weight onto the spinal cord at T 7-8 . Superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells were transplanted into the injured spinal cord via the subarachnoid space. An outer magnetic field was used to successfully guide the labeled cells to the lesion site. Prussian blue staining showed that more bone marrow mesenchymal stem cells reached the lesion site in these rats than in those without magnetic guidance or superparamagnetic iron oxide labeling, and immunofluorescence revealed a greater number of complete axons at the lesion site. Moreover, the Basso, Beattie and Bresnahan (BBB) locomotor rating scale scores were the highest in rats with superparamagnetic labeling and magnetic guidance. Our data confirm that superparamagnetic iron oxide nanoparticles effectively label bone marrow mesenchymal stem cells and impart sufficient magnetism to respond to the external magnetic field guides. More importantly, superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells can be dynamically and non-invasively tracked in vivo using magnetic resonance imaging. Superparamagnetic iron oxide labeling of bone marrow mesenchymal stem cells coupled with magnetic guidance offers a promising avenue for the clinical treatment of spinal cord injury.


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