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RESEARCH ARTICLE
Year : 2015  |  Volume : 10  |  Issue : 3  |  Page : 438-444

Neuroprotective effects of SMADs in a rat model of cerebral ischemia/reperfusion


1 Department of Neurology, First Hospital of Jilin University, Changchun, Jilin Province, China
2 Department of Respiratory Medicine, First Hospital of Jilin University, Changchun, Jilin Province, China
3 Department of Pediatrics, First Hospital of Jilin University, Changchun, Jilin Province, China
4 Department of Neurology, Affiliated Hospital of Yanbian University, Yanbian, Jilin Province, China
5 Department of Hepatic-Biliary-Pancreatic Medicine, First Hospital of Jilin University, Changchun, Jilin Province, China

Correspondence Address:
Lei Song
Department of Respiratory Medicine, First Hospital of Jilin University, Changchun, Jilin Province
China
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Source of Support: This work was supported by the National Natural Science Foundation of China, No. 81460193., Conflict of Interest: None


DOI: 10.4103/1673-5374.153693

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Previous studies have shown that up-regulation of transforming growth factor β1 results in neuroprotective effects. However, the role of the transforming growth factor β1 downstream molecule, SMAD2/3, following ischemia/reperfusion remains unclear. Here, we investigated the neuroprotective effects of SMAD2/3 by analyzing the relationships between SMAD2/3 expression and cell apoptosis and inflammation in the brain of a rat model of cerebral ischemia/reperfusion. Levels of SMAD2/3 mRNA were up-regulated in the ischemic penumbra 6 hours after cerebral ischemia/reperfusion, reached a peak after 72 hours and were then decreased at 7 days. Phosphorylated SMAD2/3 protein levels at the aforementioned time points were consistent with the mRNA levels. Over-expression of SMAD3 in the brains of the ischemia/reperfusion model rats via delivery of an adeno-associated virus containing the SMAD3 gene could reduce tumor necrosis factor-α and interleukin-1β mRNA levels, down-regulate expression of the pro-apoptotic gene, capase-3, and up-regulate expression of the anti-apoptotic protein, Bcl-2. The SMAD3 protein level was negatively correlated with cell apoptosis. These findings indicate that SMAD3 exhibits neuroprotective effects on the brain after ischemia/reperfusion through anti-inflammatory and anti-apoptotic pathways.


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