Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage
Wei Zou1, Qiu-xin Chen2, Xiao-wei Sun1, Qing-bin Chi1, Hong-yu Kuang3, Xue-ping Yu1, Xiao-hong Dai1
1 Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
2 Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
3 Department of Endocrinology, First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, China
Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province
Source of Support: This study was supported by the National Natural Science Foundation of China, No. 81273824, 30772840; Ministry of Education Doctoral Fund in China, No. 20102327110003; the Natural Science Foundation of Heilongjiang Province in China, No. ZD201204; and Special funds for Technological Innovation Research of Harbin, China, No. 2012RFXXS062, Conflict of Interest: None
Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity. To date, studies have rarely focused on changes or damage to signal transduction pathways during cerebral hemorrhage. Here, we examined the effect of acupuncture in a rat model of cerebral hemorrhage. We examined four groups: in the control group, rats received no treatment. In the model group, cerebral hemorrhage models were established by infusing non-heparinized blood into the brain. In the acupuncture group, modeled rats had Baihui (DU20) and Qubin (GB7) acupoints treated once a day for 30 minutes. In the DAPT group, modeled rats had 0.15 μg/mL DAPT solution (10 mL) infused into the brain. Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor. Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage.