Neuroprotective effect of interleukin-6 regulation of voltage-gated Na+ channels of cortical neurons is time- and dose-dependent
Wei Xia1, Guo-yi Peng2, Jiang-tao Sheng3, Fang-fang Zhu3, Jing-fang Guo2, Wei-qiang Chen M.D., Ph.D. 2
1 Department of Interventional Radiology, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China
2 Department of Neurosurgery, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China
3 Department of Pathogenic Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong Province, China
Department of Neurosurgery, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province
Source of Support: This work was supported by a grant from the National Natural Science Foundation of China, No. 30972766, 31170852, 81001322, 81172795, 81173048; and the Specialized Research Fund for the Doctoral Program of Colleges and Universities, No. 20094402110004., Conflict of Interest: None
Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour exposure of interleukin-6 on cortical neurons at various concentrations (0.1, 1, 5 and 10 ng/mL) and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations (2, 4, 8, 24 and 48 hours) by studying voltage-gated Na + channels using a patch-clamp technique. Voltage-clamp recording results demonstrated that interleukin-6 suppressed Na + currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na + channels in rat cortical neurons by interleukin-6 is time- and dose-dependent.