ORC ID ">
  • Users Online: 2278
  • Home
  • Print this page
  • Email this page
RESEARCH ARTICLE
Year : 2016  |  Volume : 11  |  Issue : 12  |  Page : 1981-1989

Construction of a plasmid for human brain-derived neurotrophic factor and its effect on retinal pigment epithelial cell viability


Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Lab, Beijing, China

Correspondence Address:
Gen-lin Li
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Lab, Beijing
China
Login to access the Email id

Source of Support: This study was supported by the National Natural Science Foundation of China, No. 81271046; the Joint Program of Beijing Municipal Natural Science Foundation (category B) and Beijing Educational Committee (key project), No. KZ201510025025., Conflict of Interest: None


DOI: 10.4103/1673-5374.197142

Rights and Permissions

Several studies have investigated the protective functions of brain-derived neurotrophic factor (BDNF) in retinitis pigmentosa. However, a BDNF-based therapy for retinitis pigmentosa is not yet available. To develop an efficient treatment for fundus disease, an eukaryotic expression plasmid was generated and used to transfect human 293T cells to assess the expression and bioactivity of BDNF on acute retinal pigment epithelial-19 (ARPE-19) cells, a human retinal epithelial cell line. After 96 hours of co-culture in a Transwell chamber, ARPE-19 cells exposed to BDNF secreted by 293T cells were more viable than ARPE-19 cells not exposed to secreted BDNF. Western blot assay showed that Bax levels were downregulated and that Bcl-2 levels were upregulated in human ARPE-19 cells exposed to BDNF. Furthermore, 293T cells transfected with the BDNF gene steadily secreted the protein. The powerful anti-apoptotic function of this BDNF may be useful for the treatment of retinitis pigmentosa and other retinal degenerative diseases.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2197    
    Printed13    
    Emailed0    
    PDF Downloaded306    
    Comments [Add]    
    Cited by others 1    

Recommend this journal