ORC ID ">
  • Users Online: 2287
  • Home
  • Print this page
  • Email this page
RESEARCH ARTICLE
Year : 2018  |  Volume : 13  |  Issue : 7  |  Page : 1281-1287

Degree of dopaminergic degeneration measured by 99mTc-TRODAT-1 SPECT/CT imaging


1 Fujian Provincial Key Laboratory of Neuroglia and Disease, Fujian Medical University; Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou, Fujian Province, China
2 Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou, Fujian Province, China

Correspondence Address:
Zhi-Hong Zheng
Fujian Provincial Key Laboratory of Neuroglia and Disease, Fujian Medical University; Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou, Fujian Province
China
Login to access the Email id

Source of Support: This study was supported by the National Natural Science Foundation of China, No. 81571250, Conflict of Interest: None


DOI: 10.4103/1673-5374.235077

Rights and Permissions

To prevent and treat Parkinson’s disease in its early stages, it is essential to be able to detect the degree of early dopaminergic neuron degeneration. Dopamine transporters (DAT) in the striatum regulate synaptic dopamine levels, and striatal 99mTc-TRODAT-1 single-photon emission computed tomography (-SPECT) imaging is a marker for presynaptic neuronal degeneration. However, the association between the degree of dopaminergic degeneration and in vivo 99mTc-TRODAT-1 SPECT imaging is unknown. Therefore, this study investigated the association between the degree of 6-hydroxydopamine (6-OHDA)-induced dopaminergic degeneration and DAT imaging using 99mTc-TRODAT-1 SPECT in rats. Different degrees of nigrostriatal dopamine depletion were generated by injecting different doses of 6-OHDA (2, 4, and 8 μg) into the right medial forebrain bundle. The degree of nigrostriatal dopaminergic neuron degeneration was assessed by rotational behavior and immunohistochemical staining. The results showed that striatal 99mTc-TRODAT-1 binding was significantly diminished both in the ipsilateral and the contralateral sides in the 4 and 8 μg 6-OHDA groups, and that DAT 99mTc-TRODAT-1 binding in the ipsilateral striatum showed a high correlation to apomorphine-induced rotations at 8 weeks post-lesion (r = –0.887, P < 0.01). There were significant correlations between DAT 99mTc-TRODAT-1 binding in the ipsilateral striatum and the amount of tyrosine hydroxylase immunoreactive neurons in the ipsilateral substantia nigra in the 2, 4, and 8 μg 6-OHDA groups at 8 weeks post-lesion (r = 0.899, P < 0.01). These findings indicate that striatal DAT imaging using 99mTc-TRODAT-1 is a useful technique for evaluating the severity of dopaminergic degeneration.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2366    
    Printed36    
    Emailed0    
    PDF Downloaded262    
    Comments [Add]    
    Cited by others 1    

Recommend this journal