ORC ID , Chang-Mei Niu2, Jia-Qi Shi2, Ying-Yu Wang3, Yu-Min Yang Ph.D. 1, Hong-Bo Wang Ph.D. 4">
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RESEARCH ARTICLE
Year : 2018  |  Volume : 13  |  Issue : 8  |  Page : 1455-1464

Novel conductive polypyrrole/silk fibroin scaffold for neural tissue repair


1 Key Laboratory of Science and Technology of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi; Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
2 Medical School, Nantong University, Nantong, Jiangsu Province, China
3 Wen Zheng College, Soochow University, Suzhou, Jiangsu Province, China
4 Key Laboratory of Science and Technology of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu Province, China

Correspondence Address:
Hong-Bo Wang
Key Laboratory of Science and Technology of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu Province
China
Yu-Min Yang
Key Laboratory of Science and Technology of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi; Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province
China
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Source of Support: This study was supported by the National Natural Science Foundation of China, No. 81671823, 81701835; a grant from the National Key Research and Development Program of China, No. 2016YFC1101603; a grant from the Natural Science Research Program of Nantong of China, No. MS12016056, Conflict of Interest: None


DOI: 10.4103/1673-5374.235303

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Three dimensional (3D) bioprinting, which involves depositing bioinks (mixed biomaterials) layer by layer to form computer-aided designs, is an ideal method for fabricating complex 3D biological structures. However, it remains challenging to prepare biomaterials with micro-nanostructures that accurately mimic the nanostructural features of natural tissues. A novel nanotechnological tool, electrospinning, permits the processing and modification of proper nanoscale biomaterials to enhance neural cell adhesion, migration, proliferation, differentiation, and subsequent nerve regeneration. The composite scaffold was prepared by combining 3D bioprinting with subsequent electrochemical deposition of polypyrrole and electrospinning of silk fibroin to form a composite polypyrrole/silk fibroin scaffold. Fourier transform infrared spectroscopy was used to analyze scaffold composition. The surface morphology of the scaffold was observed by light microscopy and scanning electron microscopy. A digital multimeter was used to measure the resistivity of prepared scaffolds. Light microscopy was applied to observe the surface morphology of scaffolds immersed in water or Dulbecco's Modified Eagle's Medium at 37°C for 30 days to assess stability. Results showed characteristic peaks of polypyrrole and silk fibroin in the synthesized conductive polypyrrole/silk fibroin scaffold, as well as the structure of the electrospun nanofiber layer on the surface. The electrical conductivity was 1 × 10−5–1 × 10−3 S/cm, while stability was 66.67%. A 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was employed to measure scaffold cytotoxicity in vitro. Fluorescence microscopy was used to observe EdU-labeled Schwann cells to quantify cell proliferation. Immunohistochemistry was utilized to detect S100β immunoreactivity, while scanning electron microscopy was applied to observe the morphology of adherent Schwann cells. Results demonstrated that the polypyrrole/silk fibroin scaffold was not cytotoxic and did not affect Schwann cell proliferation. Moreover, filopodia formed on the scaffold and Schwann cells were regularly arranged. Our findings verified that the composite polypyrrole/silk fibroin scaffold has good biocompatibility and may be a suitable material for neural tissue engineering.


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