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  Indian J Med Microbiol
 

Figure 3 Effect of CCAD on H2O2-induced cell death in neuronal-like PC12 cells. Top panel shows experimental setup. PC12 cells were seeded into 24-well plates at 30,000 cells/ well in the maintenance medium and cultivated overnight. The next day cells were treated with H2O2 (200 μM) for 2 hours. After rinse out of H2O2, cells were treated with NGF (50 ng/mL) and FGD or CCAD at 0.5 mg/mL. Cells were further cultivated for 6 days. At the end of cultivation, cell viability was measured by presto blue assay. (A) Images of PC12 cells were observed by a phase-contrast microscope. Images were taken at the end of cultivation. Magnification: 200×. Scale bars: 25 μm. (B) Results from presto blue assay. Experiments were repeated 3–5 times independently. ***P < 0.001, vs. NGF + H2O2; ††P < 0.01, vs. NGF + H2O2 + FGD (one-way analysis of variance followed by the least significant difference test). CCAD: Enzyme-digested Colla Corii Asini; FGD: enzyme-digested fish gelatin; NC: negative control; NGF: nerve growth factor.

Figure 3 Effect of CCAD on H<sub>2</sub>O<sub>2</sub>-induced cell death in neuronal-like PC12 cells.
Top panel shows experimental setup. PC12 cells were seeded into 24-well plates at 30,000 cells/ well in the maintenance medium and cultivated overnight. The next day cells were treated with H<sub>2</sub>O<sub>2</sub> (200 μM) for 2 hours. After rinse out of H<sub>2</sub>O<sub>2</sub>, cells were treated with NGF (50 ng/mL) and FGD or CCAD at 0.5 mg/mL. Cells were further cultivated for 6 days. At the end of cultivation, cell viability was measured by presto blue assay. (A) Images of PC12 cells were observed by a phase-contrast microscope. Images were taken at the end of cultivation. Magnification: 200×. Scale bars: 25 μm. (B) Results from presto blue assay. Experiments were repeated 3–5 times independently. ***<i>P</i> < 0.001, <i>vs</i>. NGF + H<sub>2</sub>O<sub>2</sub>; ††<i>P</i> < 0.01, <i>vs</i>. NGF + H<sub>2</sub>O<sub>2</sub> + FGD (one-way analysis of variance followed by the least significant difference test). CCAD: Enzyme-digested Colla <i>Corii Asini</i>; FGD: enzyme-digested fish gelatin; NC: negative control; NGF: nerve growth factor.