Figure 1: Separation of the crude S. pilchardus glycosaminoglycan extract by anion-exchange chromatography and agarose-based gel electrophoresis. (A) Anion exchange chromatogram of crude S. pilchardus glycosaminoglycan extract. Fractions 1–6 (denoted F1–6) were eluted with a NaCl gradient (dashed line) with elution monitored at λAbs of 232 nm (solid line). (B) Electrophoretic mobility of the glycosaminoglycan (GAG) extract from S. pilchardus (F4; 800 mM) compared against bona fide glycosaminoglycans of heparin, heparan sulfate (HS), chondroitin sulfate A (CSA), C (CSC) and D (CSD), and dermatan sulfate (DS). Mix = chondroitin sulfate A, heparin and heparan sulfate mixture.