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  Indian J Med Microbiol
 

Figure 1: Separation of the crude S. pilchardus glycosaminoglycan extract by anion-exchange chromatography and agarose-based gel electrophoresis. (A) Anion exchange chromatogram of crude S. pilchardus glycosaminoglycan extract. Fractions 1–6 (denoted F1–6) were eluted with a NaCl gradient (dashed line) with elution monitored at λAbs of 232 nm (solid line). (B) Electrophoretic mobility of the glycosaminoglycan (GAG) extract from S. pilchardus (F4; 800 mM) compared against bona fide glycosaminoglycans of heparin, heparan sulfate (HS), chondroitin sulfate A (CSA), C (CSC) and D (CSD), and dermatan sulfate (DS). Mix = chondroitin sulfate A, heparin and heparan sulfate mixture.

Figure 1: Separation of the crude <i>S. pilchardus</i> glycosaminoglycan extract by anion-exchange chromatography and agarose-based gel electrophoresis.
(A) Anion exchange chromatogram of crude <i>S. pilchardus</i> glycosaminoglycan extract. Fractions 1–6 (denoted F1–6) were eluted with a NaCl gradient (dashed line) with elution monitored at λ<sub>Abs</sub> of 232 nm (solid line). (B) Electrophoretic mobility of the glycosaminoglycan (GAG) extract from <i>S. pilchardus</i> (F4; 800 mM) compared against <i>bona fide</i> glycosaminoglycans of heparin, heparan sulfate (HS), chondroitin sulfate A (CSA), C (CSC) and D (CSD), and dermatan sulfate (DS). Mix = chondroitin sulfate A, heparin and heparan sulfate mixture.