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  Indian J Med Microbiol
 

Figure 6: Differential scanning fluorimetry profile for the S. pilchardus chondroitin sulfate A extract. (A) Thermal stability (first differential) profile of β-site amyloid precursor protein cleaving enzyme 1 (1 µg; black) and with 4 µg porcine mucosal heparin (green) or 4 µg S. pilchardus chondroitin sulfate A extract (blue), measured by differential scanning fluorimetry in 50 mM, pH 4.0, NaOAc buffer. ΔTm of β-site amyloid precursor protein cleaving enzyme 1 with 4 µg of porcine mucosal heparin (*** –10.5°C ± 0.5 SD, n = 3) or 4 µg S. pilchardus chondroitin sulfate A extract (** –5°C ± 1.3 SD, n = 3). (B) ΔTm of beta-site amyloid precursor protein cleaving enzyme 1 alone (black) or in the presence of increasing concentrations of porcine mucosal heparin or S. pilchardus chondroitin sulfate A extract.

Figure 6: Differential scanning fluorimetry profile for the <i>S. pilchardus</i> chondroitin sulfate A extract. 
(A) Thermal stability (first differential) profile of β-site amyloid precursor protein cleaving enzyme 1 (1 µg; black) and with 4 µg porcine mucosal heparin (green) or 4 µg <i>S. pilchardus</i> chondroitin sulfate A extract (blue), measured by differential scanning fluorimetry in 50 mM, pH 4.0, NaOAc buffer. ΔT<sub>m</sub> of β-site amyloid precursor protein cleaving enzyme 1 with 4 µg of porcine mucosal heparin (*** –10.5°C ± 0.5 SD, <i>n</i> = 3) or 4 µg <i>S. pilchardus</i> chondroitin sulfate A extract (** –5°C ± 1.3 SD, <i>n</i> = 3). (B) ΔT<sub>m</sub> of beta-site amyloid precursor protein cleaving enzyme 1 alone (black) or in the presence of increasing concentrations of porcine mucosal heparin or <i>S. pilchardus</i> chondroitin sulfate A extract.