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  Indian J Med Microbiol
 

Figure 6: Immunofluorescence staining of newborn neuron-specific DCX and Ki67 in hippocampal dentate gyrus region on day 14 post-MCAO treatment with CEGI. (A) Double staining of DCX (red, stained by Alexa Fluor®555) and Ki67 (green, stained by Alexa Fluor®488) in the ipsilateral subgranular zone of the hippocampal dentate gyrus region on day 14 post-MCAO. Scale bars: 50 μm. (B) Temporal changes in density of newborn neurons (DCX+). (C) The percentage of proliferating neurons (Ki67+/DCX+). The fluorescence intensity of DCX was significantly elevated in MCAO + 4 mL/kg CEGI group compared with that in MCAO rats (P < 0.05). The percentage of Ki67/DCX positive cells was significantly increased in CEGI-treated rats (P < 0.05). Data are presented as the mean ± SEM (n = 6 per group at each time point). *P < 0.05, vs. MCAO group (Kruskal-Wallis one-way analysis of variance followed by Dunn's post hoc test). CEGI: Cattle encephalon glycoside and ignotin; DAPI: 4',6-diamino-2-phenylindole; DCX: doublecortin; MCAO: middle cerebral artery occlusion.

Figure 6: Immunofluorescence staining of newborn neuron-specific DCX and Ki67 in hippocampal dentate gyrus region on day 14 post-MCAO treatment with CEGI. 
(A) Double staining of DCX (red, stained by Alexa Fluor®555) and Ki67 (green, stained by Alexa Fluor®488) in the ipsilateral subgranular zone of the hippocampal dentate gyrus region on day 14 post-MCAO. Scale bars: 50 μm. (B) Temporal changes in density of newborn neurons (DCX<sup>+</sup>). (C) The percentage of proliferating neurons (Ki67<sup>+</sup>/DCX<sup>+</sup>). The fluorescence intensity of DCX was significantly elevated in MCAO + 4 mL/kg CEGI group compared with that in MCAO rats (<i>P</i> < 0.05). The percentage of Ki67/DCX positive cells was significantly increased in CEGI-treated rats (<i>P</i> < 0.05). Data are presented as the mean ± SEM (<i>n</i> = 6 per group at each time point). *<i>P</i> < 0.05, <i>vs</i>. MCAO group (Kruskal-Wallis one-way analysis of variance followed by Dunn's <i>post hoc</i> test). CEGI: Cattle encephalon glycoside and ignotin; DAPI: 4',6-diamino-2-phenylindole; DCX: doublecortin; MCAO: middle cerebral artery occlusion.