Figure 6: Immunofluorescence staining of newborn neuron-specific DCX and Ki67 in hippocampal dentate gyrus region on day 14 post-MCAO treatment with CEGI. (A) Double staining of DCX (red, stained by Alexa Fluor®555) and Ki67 (green, stained by Alexa Fluor®488) in the ipsilateral subgranular zone of the hippocampal dentate gyrus region on day 14 post-MCAO. Scale bars: 50 μm. (B) Temporal changes in density of newborn neurons (DCX+). (C) The percentage of proliferating neurons (Ki67+/DCX+). The fluorescence intensity of DCX was significantly elevated in MCAO + 4 mL/kg CEGI group compared with that in MCAO rats (P < 0.05). The percentage of Ki67/DCX positive cells was significantly increased in CEGI-treated rats (P < 0.05). Data are presented as the mean ± SEM (n = 6 per group at each time point). *P < 0.05, vs. MCAO group (Kruskal-Wallis one-way analysis of variance followed by Dunn's post hoc test). CEGI: Cattle encephalon glycoside and ignotin; DAPI: 4',6-diamino-2-phenylindole; DCX: doublecortin; MCAO: middle cerebral artery occlusion.