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  Indian J Med Microbiol
 

Figure 2: CIMT increases colocalization of NR1 with GluR2 while reducing that of NR1 with GluR1 in the ipsilateral sensorimotor cortex. (A) Confocal images of NR1 (red, Cy3) and GluR2 (green, Alexa Fluor 488) double-immunolabeling in the sensorimotor cortex. Selected areas other than neuronal nuclei (with DAPI staining) were used for colocalization analysis. The right image shows a scatterplot of the correlation between NR1 and GluR2 distributions; x and y axes show values for the red and green fluorescence channels, respectively. Scale bars: 50 μm. (B) Colocalization of NR1 with GluR2 was lower in the MCAO group than in the other two groups (P < 0.01 or < 0.0001). (C) Confocal images of NR1 (red, Cy3) and GluR1 (green, Alexa Fluor 488) double-immunolabeling in the sensorimotor cortex. Selected areas other than neuronal nuclei (stained with DAPI) were used for colocalization analysis. The right image shows a scatterplot of the correlation between NR1 and GluR1 distributions; x and y axes show values for the red and green fluorescence channels, respectively. Scale bars: 50 μm. (D) Colocalization of NR1 with GluR1 was lower in the CIMT group than in the other two groups (P < 0.01 or < 0.001). Data are expressed as the mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance followed by Tukey's multiple comparisons test). CIMT: Constraint-induced movement therapy; GluR: glutamate receptor; MCAO: middle cerebral artery occlusion.

Figure 2: CIMT increases colocalization of NR1 with GluR2 while reducing that of NR1 with GluR1 in the ipsilateral sensorimotor cortex. 
(A) Confocal images of NR1 (red, Cy3) and GluR2 (green, Alexa Fluor 488) double-immunolabeling in the sensorimotor cortex. Selected areas other than neuronal nuclei (with DAPI staining) were used for colocalization analysis. The right image shows a scatterplot of the correlation between NR1 and GluR2 distributions; <i>x</i> and <i>y</i> axes show values for the red and green fluorescence channels, respectively. Scale bars: 50 μm. (B) Colocalization of NR1 with GluR2 was lower in the MCAO group than in the other two groups (<i>P</i> < 0.01 or < 0.0001). (C) Confocal images of NR1 (red, Cy3) and GluR1 (green, Alexa Fluor 488) double-immunolabeling in the sensorimotor cortex. Selected areas other than neuronal nuclei (stained with DAPI) were used for colocalization analysis. The right image shows a scatterplot of the correlation between NR1 and GluR1 distributions; x and y axes show values for the red and green fluorescence channels, respectively. Scale bars: 50 μm. (D) Colocalization of NR1 with GluR1 was lower in the CIMT group than in the other two groups  (<i>P</i> < 0.01 or < 0.001). Data are expressed as the mean ± SD. **<i>P</i> < 0.01,  ***<i>P</i> < 0.001, ****<i>P</i> < 0.0001 (one-way analysis of variance followed by Tukey's multiple comparisons test). CIMT: Constraint-induced movement therapy; GluR: glutamate receptor; MCAO: middle cerebral artery occlusion.