Figure 2: Neurological functions, brain water content, and blood-brain barrier permeability after recombinant MAP4K4 delivery and MAP4K4 silencing post SAH (Experiment II). (A) SAH grading scores. (B, C) Representative western blot bands of MAP4K4 siRNA knockdown efficiency at 24 hours post SAH. (D, E) Modified Garcia scores and beam balance test results at 24 and 72 hours. (F, G) Brain water content at 24 and 72 hours after SAH. (H) Evans blue leakage in the ipsilateral hemisphere 24 hours post SAH. Data are expressed as the mean ± SD (n = 6; one-way analysis of variance and Tukey's post hoc test). *P < 0.05, vs. sham group; #P < 0.05, vs. SAH + NS group; †P < 0.05, vs. SAH + Scr siRNA group. MAP4K4: Sterile-20-like mitogen-activated protein kinase kinase kinase kinase 4; SAH: subarachnoid hemorrhage; Scr: scramble.