Figure 6: Continuous endothelial cell and tight junction protein disruption after PF delivery post SAH (Experiment IV). Endothelium (lectin) discontinuity and tight junction (claudin 5) degradation in the ipsilateral cortex after SAH. Endothelium and tight junction discontinuity was greater in the SAH + vehicle group than in the sham group. Endothelium and tight junction discontinuity was preserved by PF administration. Scale bar: 20 μm. Arrows indicate discontinuity of endothelial cells and claudin 5. Red: claudin 5; green: lectin, blue: DAPI. DAPI: 4′,6-Diamidino-2-phenylindole; PF: PF-06260933; SAH: subarachnoid hemorrhage.