Figure 7: Protein expression of nuclear p-p65 and tight junction proteins and MMP9 activity in the ipsilateral hemisphere after PF delivery post SAH (Experiment IV). (A–D) Levels of p-p65 decreased, while the expression of ZO-1 and claudin 5 was restored after PF administration. (E, F) Representative gelatin zymography of MMP9 expression revealed that PF attenuated MMP9 expression after SAH. Data are expressed as the mean ± SD (n = 6; one-way analysis of variance and Tukey's post-hoc test).*P < 0.05, vs. sham group; #P < 0.05, vs. SAH + NS group. MMP9: Matrix metalloproteinase 9; PF: PF-06260933; SAH: subarachnoid hemorrhage.