Figure 4: Effect of TFHL on the number of Nissl bodies in neurons in the injured spinal cord. (A) The number of Nissl bodies in neurons was observed by Nissl staining in each group (n = 3; cross-section of the T10 segment of the spinal cord; scale bars: 200 μm). Blue staining represents a Nissl body, as shown by the arrows. The spinal cord morphology of each group was observed. The Nissl bodies, nucleus, and morphology of nerve cells were clearly visible in each group. (B) The number of Nissl bodies/mm2 was determined. The spinal cord injury (SCI) group had the lowest number of Nissl bodies/mm2. After 14 days of continuous treatment with 5, 10, and 20 mg/kg TFHL, the number of Nissl bodies/mm2 in the TFHL groups increased with increasing drug concentration. *P < 0.05, vs. sham operation group; #P < 0.05, vs. SCI group; †P < 0.05, vs. SCI + 5 mg/kg TFHL group. Data are expressed as the mean ± SD (one-way analysis of variance followed by a least-significant difference post hoc test). SCI: Spinal cord injury; TFHL: total flavonoids of hawthorn leaves.