Figure 5: Effect of TFHL on cell apoptosis in the injured spinal cord. (A) Apoptosis of cells was observed by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining in each group (n = 3; cross-section of the T10 segment of the spinal cord; scale bars: 200 μm). Apoptotic cells were stained by the TUNEL method, and nuclei were stained with 4′,6-diamidino-2-phenylindole. Green and blue fluorescence represent apoptotic cells and normal cells, respectively. (B) The spinal cord injury (SCI) group exhibited the largest percentage of TUNEL-positive cells. After 14 days of continuous treatment with TFHL (5, 10, and 20 mg/kg), the percentage of TUNEL-positive cells of the SCI + TFHL groups decreased with increasing drug concentration. *P < 0.05, vs. sham operation group; #P < 0.05, vs. SCI group; †P < 0.05, vs. SCI + 5 mg/kg TFHL group. Data are expressed as the mean ± SD (one-way analysis of variance followed by a least-significant difference post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; SCI: spinal cord injury; TFHL: total flavonoids of hawthorn leaves; TUNEL: terminal-deoxynucleotidyl transferase mediated nick end labeling.