Figure 6: Effect of TFHL on expression levels of Bcl-2, Bax, and cleaved caspase-3 in the injured spinal cord. (A) After 14 days of continuous treatment with TFHL (5, 10, and 20 mg/kg), the protein expression levels of Bcl-2, Bax, and cleaved caspase-3 were analyzed by western blot assays in each group (n = 6). (B–D) Gray value ratio of protein bands of Bcl-2, cleaved caspase-3, and Bax. After injury, protein expression levels of Bcl-2, Bax, and cleaved caspase-3 increased. After treatment with TFHL, the protein expression level of Bcl-2 increased, while the protein expression levels of Bax and cleaved caspase-3 decreased with increasing drug concentration in the spinal cord injury (SCI) + TFHL groups. *P < 0.05, vs. sham operation group; #P < 0.05, vs. SCI group; †P < 0.05, vs. SCI+5 mg/kg TFHL group. Data are expressed as the mean ± SD (one-way analysis of variance followed by a least-significant difference post hoc test). SCI: Spinal cord injury; TFHL: total flavonoids of hawthorn leaves.