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  Indian J Med Microbiol
 

Figure 2: In vitro study of the EGFP reporter gene expression in gene-modified leucoconcentrate GML-EGFP 72 hours after transduction of the leucoconcentrate with Ad5/35F-EGFP (multiplicity of infection = 3). (A) Fluorescent microscopy using the green channel demonstrates EGFP-positive white blood cells. (B) Flow cytometry analysis demonstrates that 0.6% of white blood cells in gene-modified leucoconcentrate (GML-EGFP) efficiently express green fluorescent protein. EGFP: Enhanced green fluorescent protein; GML: gene-modified leucoconcentrate.

Figure 2: <i>In vitro</i> study of the EGFP reporter gene expression in gene-modified leucoconcentrate GML-EGFP 72 hours after transduction of the leucoconcentrate with Ad5/35F-EGFP (multiplicity of infection = 3).
(A) Fluorescent microscopy using the green channel demonstrates EGFP-positive white blood cells. (B) Flow cytometry analysis demonstrates that 0.6% of white blood cells in gene-modified leucoconcentrate (GML-EGFP) efficiently express green fluorescent protein. EGFP: Enhanced green fluorescent protein; GML: gene-modified leucoconcentrate.