Figure 5: Localization of GABAA-ρ transcripts via in situ hybridization. Retinas from Tg(GFAP:GFP)mi2001 zebrafish expressing GFP in Müller glia were immunostained with antibodies against GFP to mark Müller glia. In situ hybridization was performed on the same sections using probes against the ρ2a subunit of the GABAA-ρ receptor (gabrr2a). (A) Z-stack formed from 69 optical slices. Optical slice thickness is 0.439 μm. (B) Representative optical slice from the z-stack in A. Scale bars: 20 μm. INL: Inner nuclear layer; OPL: outer plexiform layer. Green: GFAP:GFP; red: gabrr2a (A, B). GABAA: Gamma aminobutyric acid A; GFAP: glial fibrillary acid protein; GFP: green fluorescent protein; TPMPA: (1,2,5,6-tetrahydropyridin-4-yl)methylphosphinic acid.