Figure 3: T-cell subsets derived from splenocytes using flow cytometric analysis during the 14 days after spinal cord injury. (A, B) Percentage of gated CD4+ T-cells, following stimulation, compared with the unstimulated control. (C) Percentage of CD4+ IFN-γ+ T-helper cells, in response to stimulation. CD4+ IFN-γ+ T-cells in the spleen demonstrated a sharp decline within 3–7 days after surgery. (D) Percentage of CD4+IL17A+ T-helper cells in response to stimulation. The percentage of CD4+IL17A+ T-cells increased 7 days after surgery. (E) Percentage of CD4+IFN-γ+IL17A+ T-cells, in response to stimulation. (F, H) Percentage of gated CD4+ T-cells, compared with control. (G) Percentage of CD4+CD25+FoxP3+ regulatory T-cells. Regulatory T-cell numbers presented a sharp increase in the spleen 3 days post-injury, and then gradually declined. Data are expressed as the mean ± SEM (n = 6; one-way analysis of variance followed by Tukey's post hoc test). *P < 0.05, **P < 0.01, ***P < 0.001. FoxP3: Forkhead box P3; FSC: forward scatter; IFN-γ: interferon-γ; IL17A: interleukin 17A; SSC: side scatter.