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Figure 2: Profile of mitochondriaenrichment of miRNAs following traumatic brain injury. Animals (total n = 6 per group) were euthanatized at 1, 3, or 7 days following injury and the ipsilateral (Ipsi) and contralateral (Contra) hippocampi were rapidly removed for isolation of the mitochondria (Mito) and cytosolic (Cyto) fractions. The fractions were then subjected to RNA isolation and miRNA analysis. The level of mitochondriaenrichment of a miRNA expressed as relative quantity (RQ) was calculated using the formula RQ = 2^{–(Ct(Mito)–Ct(Cyto))} where mitochondrial and cytoplasmic fractions were isolated from the same tissue. (A) Mitochondriaenriched miRNAs. (B) Other miRNAs. Data are expressed as the mean ± SD. The difference between the ipsilateral and the contralateral hippocampi was evaluated using oneway analysis of variance and Student’s ttest. *P < 0.05, **P < 0.01. 
