Figure 3: In vitro delivery of mellitin-derived cell permeable peptide (p5RHH)-based miR-146a nanoparticles to cultured BV-2 cells. Confocal microscopy graph of miR-146a and scramble control transfections (scale bars: 10 µm) (A) and the western blot analysis of TNF receptor-associated factor 6 (TRAF6) in the transfected cells (B). Densitometry quantification of the western blot analysis from 4 independent transfection experiments (C, n = 4). Pre-seeded BV-2 cells were incubated either with miR-146a or scramble mimic/p5RHH-nanoparticle complexes or miR-146a or scramble mimic/Lipofectamine, or without carrier for 24 hours and the cells were visualized and photographed using a Nikon C2+ confocal microscope and then washed with PBS and harvested for RNA analysis or western blot analysis. The densitometry readout of TRAF6 protein signal was normalized on that of actin signal in the same protein sample. Data are expressed as the mean ± SD. The difference between miR-146a and scramble mimics was evaluated using Student’s t-test. *P < 0.05, ***P < 0.001.