Figure 4: Neuroinflammation in the SN: CD11b and GFAP immunoreactivity. (A) Representative immunostaining of CD11b (green) in C-SN and I-SN in the control and PHA/PRE groups. (B) Quantification of the expression of CD11b (% area occupied) in I-SN. (C) Representative immunostaining of GFAP (red) in C-SN and I-SN in both groups. The white dotted line represents the ROI were GFAP expression was quantified. (D) Quantification of the expression of GFAP (% area occupied) in C-SN and I-SN. Data are represented as median ± SD. n = 7/group. *P < 0.05, vs. contralateral area in the same group (Wilcoxon test). #P < 0.05 and ##P < 0.01 (Mann-Whitney U test). C-SN: Controlateral SN; GFAP: glial fibrillary acidic protein; I-SN: ipsilateral SN; SN: substantia nigra.