Figure 3: Effect of myricetin on ROS levels of rotenone-induced MES23.5 cells detected by flow cytometry. (A) Representative images of fluorometric assay for ROS levels. (B) Quantitative results of ROS levels. Rotenone (400 nM) treatment for 24 hours increased ROS production compared with the control, but was alleviated by pretreatment with 1 × 10–6 M myricetin for 1 hour. Data are presented as mean ± SD of six independent experiments. ***P < 0.001, vs. control group; #P < 0.05, vs. rotenone group (one-way analysis of variance followed by post hoc Tukey’s test). ROS: Reactive oxygen species.