Figure 5: Effect of myricetin on Fpn1 mRNA (A) and protein (B) expression in rotenone-induced MES23.5 cells. Cells were pretreated with 1 × 10–6 M myricetin for 1 hour, followed by co-treatment with 400 nM rotenone for 24 hours. Rotenone treatment downregulated Fpn1 mRNA and protein expression compared with the control. Myricetin prevented induction of Fpn1 mRNA and protein levels by rotenone. Data represent mean ± SD (n = 4 in A, 10 in B). **P < 0.01, ***P < 0.001, vs. control group; #P < 0.05, vs. rotenone group (one-way analysis of variance followed by post hoc Tukey’s test). Fpn1: Ferroportin 1; GAPDH: glyceraldehyde 3-phosphate dehydrogenase. Relative mRNA expression of Fpn1 is normalized to that of GAPDH (optical density ratio). Relative protein expression of Fpn1 is expressed as the optical density ratio to β-actin.