Figure 2: Effects of H2S on dopaminergic neurons in Parkinson’s disease mouse model. (A) Immunohistochemical staining of TH-positive neurons (green, stained by fluorescein isothiocyanate; original magnification 10×, scare bar: 40 μm). (B) Quantitative results of TH-positive neurons in the substantia nigra pars compacta (SNc). Data are expressed as mean ± SEM (n = 6). ***P < 0.001, vs. saline group; ###P < 0.001, vs. MPTP group (one-way analysis of variance followed by Tukey’s post hoc test). All samples were detected repeatedly in three independent experiments. DAPI: 4′,6-Diamidino-2-phenylindole, blue; H2S: hydrogen sulfide; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; NaHS: sodium hydrosulfide a donor of H2S; TH: tyrosine hydroxylase.