Figure 4: H2S relieves suppression of MPP+-induced adult neurogenesis in vitro. (A) Morphological observations of neurospheres in the SVZ of WT mice. Scale bar: 100 μm. (B, C) Statistical analysis showed that NaHS had no effect on the number of neurospheres, but could enlarge their diameter, which was suppressed by MPP+ stimulation. Data are expressed as mean ± SEM. **P < 0.01, vs. control group (Con); #P < 0.05, vs. MPP+ group (one-way analysis of variance followed by Tukey’s post hoc test). All experiments were repeated four independent times. H2S: Hydrogen sulfide; MPP: 1-methyl-4-phenylpyridiniumion; NaHS: sodium hydrosulfide, a donor of H2S; SVZ: subventricular zone; WT: wild type.