Figure 5: H2S restores BrdU expression in MPP+-induced primary neurospheres. (A) Immunofluorescence staining of BrdU-positive (green, stained by fluorescein isothiocyanate) proliferating adult neural stem cells (original magnification 20×, scale bar: 100 μm). (B) Quantitative data for BrdU-positive cells. Data are expressed as mean ± SEM. ***P < 0.001, vs. control group; ###P < 0.001, vs. MPP+ group (one-way analysis of variance followed by Tukey’s post hoc test). All experiments were repeated four independent times. BrdU: Bromodeoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; H2S: hydrogen sulfide; MPP: 1-methyl-4-phenylpyridiniumion; NaHS: sodium hydrosulfide, a donor of H2S.