Figure 6: H2S enhances neurogenesis by upregulating the Akt/GSK-3β/β-catenin cascade in adult neural stem cells of the SVZ. (A) Representative blots of PCNA, total-Akt, and p-Akt expression in adult neural stem cells. (B, C) Statistical analysis of PCNA and p-Akt expression in cell extracts. (D) Representative blots of GSK-3β, p-GSK-3β (Tyr216), β-catenin, and p-β-catenin (Ser37) expression in adult neural stem cells. (E, F) Statistical analysis of p-GSK-3β and p-β-catenin expression in cell extracts. Data are presented as mean ± SEM. ***P < 0.001, vs. control group; #P < 0.05, ###P < 0.001, vs. MPP+ group (one-way analysis of variance followed by Tukey’s post hoc test). All experiments were repeated four independent times. GSK-3β: Glycogen synthase kinase-3β; H2S: hydrogen sulfide; MPP: 1-methyl-4-phenylpyridiniumion; NaHS: sodium hydrosulfide, a donor of H2S; PCNA: proliferating cell nuclear antigen; SVZ: subventricular zone.