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  Indian J Med Microbiol
 

Figure 1: Heterogeneity of ocular macrophages in health and disease. (A) Embryonic pulse labeling of CX3CR1+ A2 progenitors in the Cx3cr1CreER:Rosa26-YFP line led to the identification of YFP+ (green) retinal microglia and ocular macrophages (oMac) in the cornea and ciliary body. Postnatal labeling of CX3CR1+ cells in adult mice to conditionally target long-lived resident tissue macrophages (green) allows to investigate the turnover with peripheral blood monocytes (grey), which lose their YFP-signal in the course of 4 weeks after tamoxifen administration. Under homeostatic conditions a steady replenishment was present in the cornea, negligible in the ciliary body and absent in the retina, confirmed by other constitutive active reporter mouse models, including Ccr2-RFP (red) mice labeling monocytes and Flt3Cre:Rosa26-YFP (blue) mice labeling multipotent FLT3+ hematopoietic stem cells (HSC). (B) Workflow of purifying myeloid cells by flow cytometry in the laser-induced model of choroidal neovascularization compared to controls for the subsequent analysis by single-cell RNA-seq and bioinformatics. The figure was created with BioRender.com.

<b>Figure 1: Heterogeneity of ocular macrophages in health and disease.</b>
(A) Embryonic pulse labeling of CX<sub>3</sub>CR1<sup>+</sup> A2 progenitors in the <i>Cx3cr1<sup>CreER</sup>:Rosa26-YFP</i> line led to the identification of YFP<sup>+</sup> (green) retinal microglia and ocular macrophages (oMac) in the cornea and ciliary body. Postnatal labeling of CX<sub>3</sub>CR1<sup>+</sup> cells in adult mice to conditionally target long-lived resident tissue macrophages (green) allows to investigate the turnover with peripheral blood monocytes (grey), which lose their YFP-signal in the course of 4 weeks after tamoxifen administration. Under homeostatic conditions a steady replenishment was present in the cornea, negligible in the ciliary body and absent in the retina, confirmed by other constitutive active reporter mouse models, including <i>Ccr2-RFP</i> (red) mice labeling monocytes and <i>Flt3<sup>Cre</sup>:Rosa26-YFP</i> (blue) mice labeling multipotent FLT3<sup>+</sup> hematopoietic stem cells (HSC). (B) Workflow of purifying myeloid cells by flow cytometry in the laser-induced model of choroidal neovascularization compared to controls for the subsequent analysis by single-cell RNA-seq and bioinformatics. The figure was created with BioRender.com.