Figure 6: Retrograde tracing showed fluoro-gold-labeled motoneuron number increased over time. To assess the ability of the L4-to-L4 nerve root to transmit signals, a control group (A) was designed for quantitative comparison. At 9 weeks (B), 13 weeks (C), and 17 weeks (D) after transfer, many labeled motoneurons were observed in the ventral horn, and the labeled area increased over time. (E) Quantification of fluoro-gold-labeled motoneurons. Data were expressed as the mean ± SEM (n = 10; one-way analysis of variance followed by the least significant difference post hoc test). *P < 0.05.