Figure 7: Immunofluorescence showed axon regeneration. Varying levels of immunostaining were observed in the reinnervated nerve roots collected at three time points after MAP-2 labeling. Dotted boxes indicate axon regeneration at the anastomosis site. Only partial regenerative reconnection occurred at the early stage after surgery (A and B). However, almost complete reconnection was observed at 17 weeks (C) after transfer. (D) The regenerated connectivity at the coaptation site. Data are expressed as the mean ± SEM (n = 10; one-way analysis of variance followed by the least significant difference post hoc test). *P < 0.05, **P < 0.01. MAP-2: Microtubule associated protein-2.