Figure 1: Cell locations and phenotypes sampled. (A) The region of interest used (dashed outline) for cell counts encompassed all layers of the dorsal DG, including the supra and infrapyramidal molecular layer (ML), granular cell layer (GCL) and subgranular zone (SGZ), as well as the hilus. Hoechst labels cell nuclei. Scale bar: 100 μm. (B) Schematic of the cell types quantified in this study and their relative locations within the DG. Vascular endothelial cells (red), astrocytes (yellow), radial glia-like neural stem cells (RGL-NSCs, green with single process), intermediate progenitor cells (IPCs, green, bipolar horizontal), neuroblasts/immature neurons (NB/INs, green, bipolar vertical), mature neurons (gray), oligodendroglial cells (blue), and microglia (purple). Created using BioRender.com. BrdU: Bromodeoxyuridine; CD31: cluster of differentiation 31; DCX: doublecortin; GFAP: glial fibrillary acidic protein; Iba1: ionized calcium-binding adapter molecule 1; MCM 2: minichromosome maintenance 2; NeuN: neuronal nuclear protein; Olig2: oligodendrocyte transcription factor 2; SOX2: (sex determining region Y)-box 2.