Figure 2: Representative images of immunolabeling for all cell types surveyed. (Ai–iv) Representative SOX2 and GFAP immunolabeling. Radial glia-like neural stem cells (RGL-NSCs) had SOX2+ nuclei in the subgranular zone (SGZ), GFAP+ cytoplasm, and a radial/apical process spanning the granular cell layer (Aiii, arrow). Intermediate progenitor cells (IPCs) had SOX2+ nuclei in the SGZ lacking GFAP+ cytoplasm (Aiii, chevrons). Mature astrocytes had SOX2+ nuclei, GFAP+ cytoplasm, and stellate morphology (Aiv, arrow). (Bi–iii) Minichromosome maintenance 2 (MCM2) immunolabeling and (Ci–iii) BrdU immunolabeling 2 hours post injection to reveal actively cycling cells. Orthogonal projections showing a putative RGL with colocalized BrdU+SOX2+ nucleus and GFAP+ radial process (Civ) and a putative IPC with colocalized BrdU+SOX2+ nucleus lacking GFAP (Cv). (Di–iii) Representative immunolabeling of DCX+ neuroblasts/immature neurons. (Ei–iii) NeuN immunolabeling to detect mature neurons (Fi–iii) Olig2 immunolabeling to detect oligodendroglial lineage cells. (Gi–iii) Representative Iba1 immunolabeling for microglia. (Hi–iii) CD31 immunolabeling to detect vascular endothelial cells. Scale bars represent 100 μm (i panels), 20 μm (ii panels), or 10 μm (iii–v panels). BrdU: Bromodeoxyuridine; GFAP: glial fibrillary acidic protein; SOX2: (sex determining region Y)-box 2.