Figure 3: Quantification of astroglial, neural stem and progenitor cell, neuroblast/immature and mature neuronal, oligodendroglial, microglial, and endothelial cell density in the dentate gyrus. Quantification is represented as cell density (A, C) and number of cells in a 40 μm coronal slice (B, D). Data are mean ± SEM from male (n = 5) and female (n = 4) adult mice. No significant sex difference for any cell type was detected via unpaired, two-tailed t-test (P > 0.05). (E) Matrix table showing results of one-way analysis of variance (ANOVA) with Tukey’s post hoc comparisons between cell type densities, combined across males and females, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (F) Identity of BrdU-labeled cells within the SGZ is shown as % of all BrdU-labeled cells colocalizing with the given markers. (G) Number of cells per counting frame across the anterior-posterior (A-P) extent of the dorsal dentate gyrus, divided into 3 bins by A-P coordinate, analyzed by two-way repeated measures ANOVA with Tukey’s post hoc comparisons, *P < 0.05,**P < 0.01. Astro: Astrocyte; BrdU: bromodeoxyuridine; CD31: cluster of differentiation 31; DCX: doublecortin; GFAP: glial fibrillary acidic protein; Iba1: ionized calcium-binding adapter molecule 1; IPC: intermediate progenitor cell; MCM 2: minichromosome maintenance 2; NeuN: neuronal nuclear protein; ns: not significant; Olig2: oligodendrocyte transcription factor 2; rad: radial; RGL: radial glia-like; SOX2: (sex determining region Y)-box 2; stel: stellate.