Figure 5: Effect of enriched environment on hippocampal neural stem cells through GSK-3β pathway. (A) TUNEL staining was used to detect hippocampal apoptosis (green) in fetal rats. Compared with apoptosis of the control group, apoptosis of the Sev group was increased, and apoptosis of Sev + EE group was decreased. Scale bars: 50 μm. (B, C) Western blot and real-time polymerase chain reaction were used to detect the protein and mRNA expression, respectively, of GSK-3β, β-catenin and Bcl-2 in hippocampus of offspring rats. Data are shown as mean ± SD (eight litters/group, three offspring within each litter as replicates). *P < 0.05, vs. C group; #P < 0.05, vs. Sev group (one-way analysis of variance followed by least significant difference post hoc test). DAPI: 4,6-Diamino-2-phenyl indole means nucleus; EE: environmental enrichment group; GSK-3β: glycogen synthase kinase-3β; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling.