Figure 2: RNA sequencing experiments and bioinformatics analysis of cerebral infarct areas in rats. (A) A series of strict filtering criteria was used to exclude transcripts that did share the typical characteristics of lncRNAs. (B) The number of transcripts retained after screening. The number of transcripts obtained after screening through the five steps outlined in A is shown. The X-axis indicates the screening step, and the Y-axis indicates the number of transcripts obtained after screening with the corresponding steps. (C) The number of transcripts obtained after screening and analysis with three different coding potential analysis tools: CPC2, CNCI, and Pfam. (D) LncRNA classification by type. CNCI: Coding-Non-Coding-Index; CPC2: Coding Potential Calculator-r2; lncRNA: long noncoding RNA; PFAM: Pfam Scan; TUCP: transcripts of uncertain coding potential.